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PubMed Publications| Keyword search (4,163 papers available) |  |
"Nguyen TTM" Authored Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Comparative genomic analysis of thermophilic fungi reveals convergent evolutionary adaptations and gene losses | Steindorff AS; Aguilar-Pontes MV; Robinson AJ; Andreopoulos B; LaButti K; Kuo A; Mondo S; Riley R; Otillar R; Haridas S; Lipzen A; Grimwood J; Schmutz J; Clum A; Reid ID; Moisan MC; Butler G; Nguyen TTM; Dewar K; Conant G; Drula E; Henrissat B; Hansel C; Singer S; Hutchinson MI; de Vries RP; Natvig DO; Powell AJ; Tsang A; Grigoriev IV; | 39266695 CSFG |
| 2 | Substrate specificity mapping of fungal CAZy AA3_2 oxidoreductases | Zhao H; Karppi J; Mototsune O; Poshina D; Svartström J; Nguyen TTM; Vo TM; Tsang A; Master E; Tenkanen M; | 38539167 CSFG |
| 3 | Comparative analysis of functional diversity of rumen microbiome in bison and beef heifers | Nguyen TTM; Badhan AK; Reid ID; Ribeiro G; Gruninger R; Tsang A; Guan LL; McAllister T; | 38054735 CSFG |
| 4 | Functional characterization of fungal lytic polysaccharide monooxygenases for cellulose surface oxidation | Mathieu Y; Raji O; Bellemare A; Di Falco M; Nguyen TTM; Viborg AH; Tsang A; Master E; Brumer H; | 37679837 CSFG |
| 5 | Characterization of a novel AA3_1 xylooligosaccharide dehydrogenase from Thermothelomyces myriococcoides CBS 398.93 | Zhao H; Karppi J; Nguyen TTM; Bellemare A; Tsang A; Master E; Tenkanen M; | 36476312 CSFG |
| 6 | Identification of a Novel Biosynthetic Gene Cluster in Aspergillus niger Using Comparative Genomics | Evdokias G; Semper C; Mora-Ochomogo M; Di Falco M; Nguyen TTM; Savchenko A; Tsang A; Benoit-Gelber I; | 34064722 BIOLOGY |
| 7 | Application of Transcriptomics to Compare the Carbohydrate Active Enzymes That Are Expressed by Diverse Genera of Anaerobic Fungi to Degrade Plant Cell Wall Carbohydrates. | Gruninger RJ, Nguyen TTM, Reid ID, Yanke JL, Wang P, Abbott DW, Tsang A, McAllister T | 30061875 CSFG |
| 8 | Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger. | Song L, Ouedraogo JP, Kolbusz M, Nguyen TTM, Tsang A | 30142205 CSFG |
| 9 | The gold-standard genome of Aspergillus niger NRRL 3 enables a detailed view of the diversity of sugar catabolism in fungi. | Aguilar-Pontes MV, Brandl J, McDonnell E, Strasser K, Nguyen TTM, Riley R, Mondo S, Salamov A, Nybo JL, Vesth TC, Grigoriev IV, Andersen MR, Tsang A, de Vries RP | 30425417 CSFG |
| 10 | Genomic and exoproteomic diversity in plant biomass degradation approaches among Aspergilli | Mäkelä MR; DiFalco M; McDonnell E; Nguyen TTM; Wiebenga A; Hildén K; Peng M; Grigoriev IV; Tsang A; de Vries RP; | 30487660 CSFG |
| Title: | Characterization of a novel AA3_1 xylooligosaccharide dehydrogenase from Thermothelomyces myriococcoides CBS 398.93 | ||||
| Authors: | Zhao H, Karppi J, Nguyen TTM, Bellemare A, Tsang A, Master E, Tenkanen M | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/36476312/ | ||||
| DOI: | 10.1186/s13068-022-02231-w | ||||
| Publication: | Biotechnology for biofuels and bioproducts | ||||
| Keywords: | AA3_1; CAZy AA3; Cellobiose dehydrogenase; Thermothelomyces myriococcoides; Xylooligosaccharide dehydrogenase; | ||||
| PMID: | 36476312 | Category: | Date Added: | 2022-12-08 | |
| Dept Affiliation: |
CSFG
1 Department of Food and Nutrition, University of Helsinki, Helsinki, Finland. hongbo.zhao@helsinki.fi. 2 Department of Food and Nutrition, University of Helsinki, Helsinki, Finland. 3 Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6, Canada. 4 Department of Bioproducts and Biosystems, Aalto University, Espoo, Finland. 5 Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada. |
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Description: |
Background: The Carbohydrate-Active enZymes (CAZy) auxiliary activity family 3 (AA3) comprises flavin adenine dinucleotide-dependent (FAD) oxidoreductases from the glucose-methanol-choline (GMC) family, which play auxiliary roles in lignocellulose conversion. The AA3 subfamily 1 predominantly consists of cellobiose dehydrogenases (CDHs) that typically comprise a dehydrogenase domain, a cytochrome domain, and a carbohydrate-binding module from family 1 (CBM1). Results: In this work, an AA3_1 gene from T. myriococcoides CBS 398.93 encoding only a GMC dehydrogenase domain was expressed in Aspergillus niger. Like previously characterized CDHs, this enzyme (TmXdhA) predominantly accepts linear saccharides with ß-(1 ? 4) linkage and targets the hydroxyl on the reducing anomeric carbon. TmXdhA was distinguished, however, by its preferential activity towards xylooligosaccharides over cellooligosaccharides. Amino acid sequence analysis showed that TmXdhA possesses a glutamine at the substrate-binding site rather than a threonine or serine that occupies this position in previously characterized CDHs, and structural models suggest the glutamine in TmXdhA could facilitate binding to pentose sugars. Conclusions: The biochemical analysis of TmXdhA revealed a catalytic preference for xylooligosaccharide substrates. The modeled structure of TmXdhA provides a reference for the screening of oxidoreductases targeting xylooligosaccharides. We anticipate TmXdhA to be a good candidate for the conversion of xylooligosaccharides to added-value chemicals by its exceptional catalytic ability. |
