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PubMed Publications| Keyword search (4,163 papers available) |  |
"Murphy J" Authored Publications:
| Title: | Senescence markers in subcutaneous preadipocytes differ in childhood- versus adult-onset obesity before and after weight loss | ||||
| Authors: | Murphy J, Tam BT, Kirkland JL, Tchkonia T, Giorgadze N, Pirtskhalava T, Tsoukas MA, Morais JA, Santosa S | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/37194560/ | ||||
| DOI: | 10.1002/oby.23745 | ||||
| Publication: | Obesity (Silver Spring, Md.) | ||||
| Keywords: | |||||
| PMID: | 37194560 | Category: | Date Added: | 2023-05-17 | |
| Dept Affiliation: |
PERFORM
1 Department of Health, Kinesiology, and Applied Physiology, Concordia University, Montréal, Québec, Canada. 2 Metabolism, Obesity, and Nutrition Laboratory, PERFORM Centre, Concordia University, Montréal, Québec, Canada. 3 Centre de recherche-Axe maladies chroniques, Centre intégré universitaire de santé et de services sociaux du Nord-de-l'Ile-de-Montréal, Hôpital du Sacré-Coeur de Montréal, Montréal, Québec, Canada. 4 Department of Sport, Physical Education, and Health, Faculty of Social Sciences, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. 5 Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, Minnesota, USA. 6 Division of Endocrinology and Metabolism, Department of Medicine, McGill University, Royal Victoria Hospital, McGill University Health Centre Glen Site, Montréal, Québec, Canada. 7 Division of Geriatric Medicine, Department of Medicine, McGill University, McGill University Health Centre-Montréal General Hospital, Montréal, Québec, Canada. |
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Description: |
Objective: The aim of this study was to determine the effect of age of obesity onset on senescence-related markers in abdominal (AB) and femoral (FEM) subcutaneous adipose tissue (SAT) before and after moderate (~10%) weight loss. Methods: AB and FEM SAT were collected from human females with childhood-onset obesity (CO) or adult-onset obesity (AO) before and after diet- and exercise-induced weight loss. Immunofluorescence analysis of ?H2AX/RAD51 (DNA damage/repair markers) and p53/p21 (senescence markers) was conducted in cultured preadipocytes, and senescence-associated ß-galactosidase (SA-ß-gal) activity was measured in SAT. Results: CO had proportionately more AB and FEM preadipocytes with DNA damage (?H2AX+ ) and senescence markers (p53+ and/or p21+ ) than AO at baseline. The proportion of ?H2AX+ FEM preadipocytes declined with weight loss in CO and was similar between groups after weight loss. The number of ?H2AX foci in ?H2AX+ preadipocytes decreased similarly between groups and regions with weight loss in parallel with an increase in RAD51. The proportion of p53+ and p21+ preadipocytes and SA-ß-gal+ cells in SAT did not change with weight loss, but the total p21 intensity in p53+ /p21+ FEM preadipocytes declined in AO. Conclusions: These results provide preliminary evidence that females with CO have an accelerated preadipocyte aging state that improves with weight loss in terms of DNA damage but not senescence. |
