Keyword search (4,163 papers available)

"McNally EK" Authored Publications:

Title Authors PubMed ID
1 A two-tiered system for selective receptor and transporter protein degradation Golden CK; Kazmirchuk TDD; McNally EK; El Eissawi M; Gokbayrak ZD; Richard JD; Brett CL; 36215320
BIOLOGY
2 Rab-Effector-Kinase Interplay Modulates Intralumenal Fragment Formation during Vacuole Fusion. Karim MA, McNally EK, Samyn DR, Mattie S, Brett CL 30269949
BIOLOGY
3 The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation. McNally EK, Brett CL 30560896
BIOLOGY

 

Title:The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation.
Authors:McNally EK,  Brett CL
Link:https://www.ncbi.nlm.nih.gov/pubmed/30560896?dopt=Abstract
DOI:10.1038/s41467-018-07734-5
Publication:Nature communications
Keywords:
PMID:30560896 Category:Nat Commun Date Added:2019-06-07
Dept Affiliation: BIOLOGY
1 Department of Biology, Concordia University, 7141 Sherbrooke St. W., SP-501.15, Montréal, QC, H4R 1R6, Canada.
2 Department of Biology, Concordia University, 7141 Sherbrooke St. W., SP-501.15, Montréal, QC, H4R 1R6, Canada. christopher.brett@concordia.ca.

Description:

The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation.

Nat Commun. 2018 12 18;9(1):5358

Authors: McNally EK, Brett CL

Abstract

Surface receptor and transporter protein down-regulation is assumed to be exclusively mediated by the canonical multivesicular body (MVB) pathway and ESCRTs (Endosomal Sorting Complexes Required for Transport). However, few surface proteins are known to require ESCRTs for down-regulation, and reports of ESCRT-independent degradation are emerging, suggesting that alternative pathways exist. Here, using Saccharomyces cerevisiae as a model, we show that the hexose transporter Hxt3 does not require ESCRTs for down-regulation conferring resistance to 2-deoxyglucose. This is consistent with GFP-tagged Hxt3 bypassing ESCRT-mediated entry into intralumenal vesicles at endosomes. Instead, Hxt3-GFP accumulates on vacuolar lysosome membranes and is sorted into an area that, upon fusion, is internalized as an intralumenal fragment (ILF) and degraded. Moreover, heat stress or cycloheximide trigger degradation of Hxt3-GFP and other surface transporter proteins (Itr1, Aqr1) by this ESCRT-independent process. How this ILF pathway compares to the MVB pathway and potentially contributes to physiology is discussed.

PMID: 30560896 [PubMed - indexed for MEDLINE]




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