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PubMed Publications| Keyword search (4,164 papers available) |  |
"McCallum G" Authored Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | A Bacteroides synthetic biology toolkit to build an in vivo malabsorption biosensor | McCallum G; Burckhardt JC; He J; Hong A; Potvin-Trottier L; Tropini C; | 41610848 BIOLOGY |
| 2 | Measuring prion propagation in single bacteria elucidates mechanism of loss | Jager K; Orozco-Hidalgo MT; Springstein BL; Joly-Smith E; Papazotos F; McDonough E; Fleming E; McCallum G; Hilfinger A; Hochschild A; Potvin-Trottier L; | 36712035 BIOLOGY |
| 3 | The gut microbiota and its biogeography | McCallum G; Tropini C; | 37740073 ENCS |
| 4 | Measuring prion propagation in single bacteria elucidates a mechanism of loss | Jager K; Orozco-Hidalgo MT; Springstein BL; Joly-Smith E; Papazotos F; McDonough E; Fleming E; McCallum G; Yuan AH; Hilfinger A; Hochschild A; Potvin-Trottier L; | 37738299 PHYSICS |
| 5 | Phylogenomic fingerprinting of tempo and functions of horizontal gene transfer within ochrophytes. | Dorrell RG, Villain A, Perez-Lamarque B, Audren de Kerdrel G, McCallum G, Watson AK, Ait-Mohamed O, Alberti A, Corre E, Frischkorn KR, Pierella Karlusich JJ, Pelletier E, Morlon H, Bowler C, Blanc G | 33419955 BIOLOGY |
| 6 | Using Models to (Re-)Design Synthetic Circuits. | McCallum G, Potvin-Trottier L | 33405217 BIOLOGY |
| Title: | A Bacteroides synthetic biology toolkit to build an in vivo malabsorption biosensor | ||||
| Authors: | McCallum G, Burckhardt JC, He J, Hong A, Potvin-Trottier L, Tropini C | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/41610848/ | ||||
| DOI: | 10.1016/j.cell.2025.12.052 | ||||
| Publication: | Cell | ||||
| Keywords: | Bacteroides; Bacteroides thetaiotaomicron transcriptomics; commensal bacterium synthetic biology; fluorescent protein reporters; gut biosensor; inducible promoters; malabsorption; repressor sponging; transcriptional reporters; | ||||
| PMID: | 41610848 | Category: | Date Added: | 2026-01-30 | |
| Dept Affiliation: |
BIOLOGY
1 Department of Microbiology & Immunology, University of British Columbia, Vancouver, BC, Canada. 2 School of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada. 3 Department of Biology, Concordia University, Montréal, QC, Canada; Department of Physics, Concordia University, Montréal, QC, Canada; Center for Applied Synthetic Biology, Concordia University, Montréal, QC, Canada. 4 Department of Microbiology & Immunology, University of British Columbia, Vancouver, BC, Canada; School of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada; Humans and the Microbiome Program, Canadian Institute for Advanced Research (CIFAR), Toronto, ON, Canada. Electronic address: carolina.tropini@ubc.ca. |
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Description: |
The human gut is a dynamic environment, where changes in pH, oxygen, and osmolality influence microbiota composition and disease. Monitoring these environmental shifts is crucial for advancing gut health diagnostics and therapeutics, yet non-invasive monitoring tools remain limited. Genetically tractable commensals, including Bacteroides thetaiotaomicron, offer promising chassis for engineering biosensors but lack modular systems for precise sensing and reporting. Here, we developed genetic tools for B. thetaiotaomicron, including (1) repressible promoters for tunable fluorescent protein expression, (2) a DNA-based system to modulate repressor activity, (3) a modular, fluorescence-based transcriptional reporter circuit, and (4) an alternative plasmid integration mode. Using these components, we engineered biosensors to detect increased gut osmolality caused by malabsorption and validated them in vitro and in a murine model of laxative-induced osmotic diarrhea. These biosensors enabled long-term, non-invasive reporting of gut osmolality from single-cell fluorescence, demonstrating the potential of gut bacteria as monitoring platforms in gut health applications. |
