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Title		Authors	PubMed ID
1	Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics	Gruninger RJ; Tsang A; McAllister TA;	37149538 CSFG
2	Effect of ammonia fiber expansion-treated wheat straw and a recombinant fibrolytic enzyme on rumen microbiota and fermentation parameters, total tract digestibility, and performance of lambs.	Ribeiro GO; Gruninger RJ; Jones DR; Beauchemin KA; Yang WZ; Wang Y; Abbott DW; Tsang A; McAllister TA;	32369600 CSFG
3	Effects of a recombinant fibrolytic enzyme on fiber digestion, ruminal fermentation, nitrogen balance and total tract digestibility of heifers fed a high forage diet.	Ran T, Saleem AM, Shen Y, Ribeiro GO, Beauchemin KA, Tsang A, Yang W, McAllister TA	31251799 CSFG
4	The production and characterization of a new active lipase from Acremonium alcalophilum using a plant bioreactor.	Pereira EO, Tsang A, McAllister TA, Menassa R	23915965 CSFG
5	Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting.	Badhan A, Wang YX, Gruninger R, Patton D, Powlowski J, Tsang A, McAllister TA	26180803 CSFG
6	Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics.	Gruninger RJ, Reid I, Forster RJ, Tsang A, McAllister TA	28417376 CSFG
7	Discovery and characterization of family 39 glycoside hydrolases from rumen anaerobic fungi with polyspecific activity on rare arabinosyl substrates.	Jones DR, Uddin MS, Gruninger RJ, Pham TTM, Thomas D, Boraston AB, Briggs J, Pluvinage B, McAllister TA, Forster RJ, Tsang A, Selinger LB, Abbott DW	28588026 CSFG
8	Identification of novel enzymes to enhance the ruminal digestion of barley straw	Badhan A; Ribeiro GO; Jones DR; Wang Y; Abbott DW; Di Falco M; Tsang A; McAllister TA;	29621684 CSFG
9	New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw.	Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA	30053012 CSFG

Title:	Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics.
Authors:	Gruninger RJ, Reid I, Forster RJ, Tsang A, McAllister TA
Link:	https://www.ncbi.nlm.nih.gov/pubmed/28417376?dopt=Abstract
DOI:	10.1007/978-1-4939-6899-2_21
Publication:	Methods in molecular biology (Clifton, N.J.)
Keywords:	Carbohydrate active enzyme; Fungi; Neocallimastigomycota; RNA-Seq; Transcriptomics;
PMID:	28417376	Category:	Methods Mol Biol	Date Added:	2019-06-07
Dept Affiliation:	CSFG 1 Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 5403 1st Ave. South, Lethbridge, AB, Canada, T1J 4B1. robert.gruninger@agr.gc.ca. 2 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada. 3 Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 5403 1st Ave. South, Lethbridge, AB, Canada, T1J 4B1.

Description:

Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics.

Methods Mol Biol. 2017;1588:279-298

Authors: Gruninger RJ, Reid I, Forster RJ, Tsang A, McAllister TA

Abstract

Lignocellulosic biomass represents an abundant, renewable resource that can be used to produce biofuels, low-cost livestock feed, and high-value chemicals. The potential of this resource has led to intensive research efforts to develop cost effective methods to breakdown lignocellulose. The efficiency with which the anaerobic fungi (phylum Neocallimastigomycota) degrade plant biomass is well recognized and in recent years has received renewed interest. Transcriptomics has been used to identify enzymes that are expressed by these fungi and are involved in the degradation of a range of lignocellulose feedstocks. The transcriptome is the entire complement of coding and noncoding RNA transcripts that are expressed by a cell under a particular set of conditions. Monitoring changes in gene expression can provide fundamental information about the biology of an organism. Here we outline a general methodology that will enable researchers to conduct comparative transcriptomic studies with the goal of identifying enzymes involved in the degradation of the plant cell wall. The method described here includes growth of fungal cultures, isolation and sequencing of RNA, and a basic description of data analysis for bioinformatic identification of differentially expressed transcripts.

PMID: 28417376 [PubMed - indexed for MEDLINE]

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