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PubMed Publications| Keyword search (4,163 papers available) |  |
"Little SR" Authored Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Correction: Miniaturized scalable arrayed CRISPR screening in primary cells enables discovery at the single donor resolution | Patel MA; Boribong BP; Sinha H; Xiao B; Xie K; Vo PQN; Chin AB; Ellouzi A; Little SR; Shih SCC; Wu H; Muller WJ; Hirukawa A; | 41028230 BIOLOGY |
| 2 | Miniaturized scalable arrayed CRISPR screening in primary cells enables discovery at the single donor resolution | Patel MA; Boribong BP; Sinha H; Xiao B; Xie K; Vo PQN; Chin AB; Ellouzi A; Little SR; Shih S; Wu H; Muller WJ; Hirukawa A; | 40790054 BIOLOGY |
| 3 | Modulatory effects of M3 muscarinic acetylcholine receptor on inflammatory profiles of human memory T helper cells | Gholizadeh F; Hajiaghayi M; Choi JS; Little SR; Rahbari N; Kargar M; Brotto K; Han E; Shih SCC; Darlington PJ; | 40405417 BIOLOGY |
| 4 | A Digital Microfluidic Platform for the Microscale Production of Functional Immune Cell Therapies | Little SR; Rahbari N; Hajiaghayi M; Gholizadeh F; Cloarec-Ung FM; Phillips J; Sinha H; Hirukawa A; Knapp DJHF; Darlington PJ; Shih SCC; | 40390294 BIOLOGY |
| 5 | The β2-adrenergic biased agonist nebivolol inhibits the development of Th17 and the response of memory Th17 cells in an NF-κB-dependent manner | Hajiaghayi M; Gholizadeh F; Han E; Little SR; Rahbari N; Ardila I; Lopez Naranjo C; Tehranimeh K; Shih SCC; Darlington PJ; | 39445009 BIOLOGY |
| 6 | An Automated Single-Cell Droplet-Digital Microfluidic Platform for Monoclonal Antibody Discovery | Ahmadi F; Tran H; Letourneau N; Little SR; Fortin A; Moraitis AN; Shih SCC; | 38441226 BIOLOGY |
| 7 | An electrochemical aptasensor for Δ9-tetrahydrocannabinol detection in saliva on a microfluidic platform | Kékedy-Nagy L; Perry JM; Little SR; Llorens OY; Shih SCC; | 36549107 BIOLOGY |
| 8 | Viral Generation, Packaging, and Transduction on a Digital Microfluidic Platform | Quach ABV; Little SR; Shih SCC; | 35192339 BIOLOGY |
| Title: | An Automated Single-Cell Droplet-Digital Microfluidic Platform for Monoclonal Antibody Discovery | ||||
| Authors: | Ahmadi F, Tran H, Letourneau N, Little SR, Fortin A, Moraitis AN, Shih SCC | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/38441226/ | ||||
| DOI: | 10.1002/smll.202308950 | ||||
| Publication: | Small (Weinheim an der Bergstrasse, Germany) | ||||
| Keywords: | antibody discovery; deterministic co-encapsulation; droplet operations; droplet-based microfluidics; | ||||
| PMID: | 38441226 | Category: | Date Added: | 2024-03-05 | |
| Dept Affiliation: |
BIOLOGY
1 Department of Electrical and Computer Engineering, Concordia University, 1455 de Maisonneuve Blvd. West, Montréal, Québec, H3G 1M8, Canada. 2 Centre for Applied Synthetic Biology, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec, H4B 1R6, Canada. 3 Department of Biology, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec, H4B 1R6, Canada. 4 Human Health Therapeutics Research Centre, National Research Council Canada, Montréal, Québec, H4P 2R2, Canada. |
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Description: |
Monoclonal antibody (mAb) discovery plays a prominent role in diagnostic and therapeutic applications. Droplet microfluidics has become a standard technology for high-throughput screening of antibody-producing cells due to high droplet single-cell confinement frequency and rapid analysis and sorting of the cells of interest with their secreted mAbs. In this work, a new method is described for on-demand co-encapsulation of cells that eliminates the difficulties associated with washing in between consecutive steps inside the droplets and enables the washing and addition of fresh media. The new platform identifies hybridoma cells that are expressing antibodies of interest using antibody-characterization assays to find the best-performing or rare-cell antibody candidates. |
