Keyword search (4,163 papers available)

"Kwan DH" Authored Publications:

Title Authors PubMed ID
1 Functional and structural characterization of an IclR family transcription factor for the development of dicarboxylic acid biosensors Pham C; Nasr MA; Skarina T; Di Leo R; Kwan DH; Martin VJJ; Stogios PJ; Mahadevan R; Savchenko A; 38696354
BIOLOGY
2 Divergent directed evolution of a TetR-type repressor towards aromatic molecules Nasr MA; Martin VJJ; Kwan DH; 37377432
BIOLOGY
3 A "biphasic glycosyltransferase high-throughput screen" identifies novel anthraquinone glycosides in the diversification of phenolic natural products Mohideen FI; Kwan DH; 36682498
CHEMBIOCHEM
4 Engineering the Enzyme Toolbox to Tailor Glycosylation in Small Molecule Natural Products and Protein Biologics Ouadhi S; López DMV; Mohideen FI; Kwan DH; 36444941
ENCS
5 A Versatile Transcription Factor Biosensor System Responsive to Multiple Aromatic and Indole Inducers Nasr MA; Timmins LR; Martin VJJ; Kwan DH; 35316041
CHEMBIOCHEM
6 In Vitro Reconstitution of the dTDP-l-Daunosamine Biosynthetic Pathway Provides Insights into Anthracycline Glycosylation Mohideen FI; Nguyen LH; Richard JD; Ouadhi S; Kwan DH; 34751552
CHEMBIOCHEM
7 Resources and Methods for Engineering "Designer" Glycan-Binding Proteins. Warkentin R, Kwan DH 33450899
CHEMBIOCHEM
8 Enzymatic Synthesis of a Fluorogenic Reporter Substrate and the Development of a High-Throughput Assay for Fucosyltransferase VIII Provide a Toolkit to Probe and Inhibit Core Fucosylation. Soroko M, Kwan DH 32441090
CHEMBIOCHEM
9 Structure-Guided Directed Evolution of Glycosidases: A Case Study in Engineering a Blood Group Antigen-Cleaving Enzyme. Kwan DH 28935105
CSFG

 

Title:Structure-Guided Directed Evolution of Glycosidases: A Case Study in Engineering a Blood Group Antigen-Cleaving Enzyme.
Authors:Kwan DH
Link:https://www.ncbi.nlm.nih.gov/pubmed/28935105?dopt=Abstract
DOI:10.1016/bs.mie.2017.06.002
Publication:Methods in enzymology
Keywords:Blood group antigen;  Directed evolution;  Enzymatic oligosaccharide synthesis;  Enzyme engineering;  Glycosidase;  High-throughput assays;  Semirational design; 
PMID:28935105 Category:Methods Enzymol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Centre for Applied Synthetic Biology, Centre for Structural and Functional Genomics, Concordia University, Montréal, Québec, Canada. Electronic address: david.kwan@concordia.ca.

Description:

Structure-Guided Directed Evolution of Glycosidases: A Case Study in Engineering a Blood Group Antigen-Cleaving Enzyme.

Methods Enzymol. 2017;597:25-53

Authors: Kwan DH

Abstract

Directed evolution is an incredibly powerful strategy for engineering enzyme function. Applying this approach to glycosidases offers enormous potential for the development of highly specialized tools in chemical glycobiology. Performing enzyme directed evolution requires the generation, by random mutagenesis, of mutant libraries from which large numbers of variant enzymes must be screened in high-throughput assays. A structure-guided "semirational" method for library creation allows researchers to target specific amino acid positions for mutagenesis, concentrating mutations where they might be most effective in order to produce mutant libraries of a manageable size, minimizing screening effort while maximizing the chances of finding improved mutants. Well-designed assays, which may use specially prepared substrates, enable efficient screening of these mutant libraries. This chapter will detail general methods in the structure-guided directed evolution of glycosidases, which have previously been employed in engineering a blood group antigen-cleaving enzyme.

PMID: 28935105 [PubMed - indexed for MEDLINE]




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