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PubMed Publications| Keyword search (4,163 papers available) |  |
"Jones DR" Authored Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Effect of ammonia fiber expansion-treated wheat straw and a recombinant fibrolytic enzyme on rumen microbiota and fermentation parameters, total tract digestibility, and performance of lambs. | Ribeiro GO; Gruninger RJ; Jones DR; Beauchemin KA; Yang WZ; Wang Y; Abbott DW; Tsang A; McAllister TA; | 32369600 CSFG |
| 2 | Discovery and characterization of family 39 glycoside hydrolases from rumen anaerobic fungi with polyspecific activity on rare arabinosyl substrates. | Jones DR, Uddin MS, Gruninger RJ, Pham TTM, Thomas D, Boraston AB, Briggs J, Pluvinage B, McAllister TA, Forster RJ, Tsang A, Selinger LB, Abbott DW | 28588026 CSFG |
| 3 | Identification of novel enzymes to enhance the ruminal digestion of barley straw | Badhan A; Ribeiro GO; Jones DR; Wang Y; Abbott DW; Di Falco M; Tsang A; McAllister TA; | 29621684 CSFG |
| Title: | Identification of novel enzymes to enhance the ruminal digestion of barley straw | ||||
| Authors: | Badhan A, Ribeiro GO, Jones DR, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister TA | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/29621684/ | ||||
| DOI: | 10.1016/j.biortech.2018.03.086 | ||||
| Publication: | Bioresource technology | ||||
| Keywords: | Blue Native PAGE; Carbohydrate active enzymes (CAZymes); Fiber utilization; Glycosyl hydrolase; LC-MS/MS; Rumen nutrition; | ||||
| PMID: | 29621684 | Category: | Bioresour Technol | Date Added: | 2019-06-07 |
| Dept Affiliation: |
CSFG
1 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta T1J 4P4, Canada. 2 Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec H4B 1R6, Canada. 3 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta T1J 4P4, Canada. Electronic address: tim.mcallister@agr.gc.ca. |
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Description: |
Crude enzyme extracts typically contain a broad spectrum of enzyme activities, most of which are redundant to those naturally produced by the rumen microbiome. Identification of enzyme activities that are synergistic to those produced by the rumen microbiome could enable formulation of enzyme cocktails that improve fiber digestion in ruminants. Compared to untreated barley straw, Viscozyme® increased gas production, dry matter digestion (P < 0.01) and volatile fatty acid production (P < 0.001) in ruminal batch cultures. Fractionation of Viscozyme® by Blue Native PAGE and analyses using a microassay and mass-spectrometry revealed a GH74 endoglucanase, GH71 a-1,3-glucanase, GH5 mannanase, GH7 cellobiohydrolase, GH28 pectinase, and esterases from Viscozyme® contributed to enhanced saccharification of barley straw by rumen mix enzymes. Grouping of these identified activities with their carbohydrate active enzymes (CAZy) counterparts enabled selection of similar CAZymes for downstream production and screening. Mining of these specific activities from other biological systems could lead to high value enzyme formulations for ruminants. |
