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PubMed Publications| Keyword search (4,163 papers available) |  |
"Jiang H" Authored Publications:
| Title: | Evaluation of D10-Leu metabolic labeling coupled with MALDI-MS analysis in studying the response of the yeast proteome to H2O2 challenge | ||||
| Authors: | Jiang H, English AM | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/17022625/ | ||||
| DOI: | 10.1021/pr060019m | ||||
| Publication: | Journal of proteome research | ||||
| Keywords: | |||||
| PMID: | 17022625 | Category: | J Proteome Res | Date Added: | 2019-06-19 |
| Dept Affiliation: |
CBAMS
1 Centre for Biological Applications of Mass Spectrometry, Department of Chemistry and Biochemistry, Concordia University, Montreal, Quebec, Canada H4B 1R6. |
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Description: |
An efficient D10-Leu metabolic-labeling method combined with isotope-ratio quantitation by MALDI-TOF MS was used to probe the response of the yeast proteome to H2O2. Control cultures correct for effects not associated with H2O2 challenge. A stress-response index to H2O2 (SRIH2O2) is defined, and values are reported for seven proteins at 45-225 min following exposure to 0.4 mM H2O2. The time course of protein accumulation in unstressed cells following the H10- to D10-SCD switch suggests that proteome responses at <45 min could be monitored by addition of excess D10-Leu to H10-cultures. |
