Keyword search (4,163 papers available)

"Huang J" Authored Publications:

Title Authors PubMed ID
1 Guanidinylated cluster-modified chitosan for wet-strength paper Gu J; Gu Z; Wu B; Xiao S; Zheng S; Chen N; Zhuang J; Liu H; Jia Z; Meng Y; Cui X; Huang L; 40947212
ENCS
2 Early family socioeconomic status and asthma-related outcomes in school-aged children: Results from seven birth cohort studies Yang-Huang J; McGrath JJ; Gauvin L; Nikiéma B; Spencer NJ; Awad YA; Clifford S; Markham W; Mensah F; Andersson White P; Ludvigsson J; Faresjö T; Duijts L; van Grieken A; Raat H; 38849153
PERFORM
3 Discovery and preclinical development of a therapeutically active nanobody-based chimeric antigen receptor targeting human CD22 McComb S; Arbabi-Ghahroudi M; Hay KA; Keller BA; Faulkes S; Rutherford M; Nguyen T; Shepherd A; Wu C; Marcil A; Aubry A; Hussack G; Pinto DM; Ryan S; Raphael S; van Faassen H; Zafer A; Zhu Q; Maclean S; Chattopadhyay A; Gurnani K; Gilbert R; Gadoury C; Iqbal U; Fatehi D; Jezierski A; Huang J; Pon RA; Sigrist M; Holt RA; Nelson BH; Atkins H; Kekre N; Yung E; Webb J; Nielsen JS; Weeratna RD; 38596311
BIOLOGY
4 Reinforcement learning for automatic quadrilateral mesh generation: A soft actor-critic approach Pan J; Huang J; Cheng G; Zeng Y; 36375347
ENCS
5 Household income and maternal education in early childhood and risk of overweight and obesity in late childhood: Findings from seven birth cohort studies in six high-income countries White PA; Awad YA; Gauvin L; Spencer NJ; McGrath JJ; Clifford SA; Nikiema B; Yang-Huang J; Goldhaber-Fiebert JD; Markham W; Mensah FK; van Grieken A; Raat H; Jaddoe VWV; Ludvigsson J; Faresjö T; 35821522
PERFORM
6 Maturation of temporal saccade prediction from childhood to adulthood: predictive saccades, reduced pupil size and blink synchronization Calancie OG; Brien DC; Huang J; Coe BC; Booij L; Khalid-Khan S; Munoz DP; 34759032
PSYCHOLOGY
7 Exploring the use of ceramic disk filter coated with Ag/ZnO nanocomposites as an innovative approach for removing Escherichia coli from household drinking water. Huang J, Huang G, An C, Xin X, Chen X, Zhao Y, Feng R, Xiong W 31864067
ENCS
8 Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi. Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T 29803771
CSFG
9 New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw. Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA 30053012
CSFG
10 Performance of ceramic disk filter coated with nano ZnO for removing Escherichia coli from water in small rural and remote communities of developing regions. Huang J, Huang G, An C, He Y, Yao Y, Zhang P, Shen J 29544196
ENCS

 

Title:Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi.
Authors:Badhan AHuang JWang YAbbott DWDi Falco MTsang AMcAllister T
Link:https://www.ncbi.nlm.nih.gov/pubmed/29803771?dopt=Abstract
DOI:10.1016/j.nbt.2018.05.003
Publication:New biotechnology
Keywords:Aerobic fungiBarley strawBlue native PAGECarbohydrate active enzymesGlycosyl hydrolaseMulti-enzyme complex
PMID:29803771 Category:N Biotechnol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta, Canada.
2 Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, 330096, China.
3 Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec, H4B 1R6, Canada.
4 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta, Canada. Electronic address: tim.mcallister@agr.gc.ca.

Description:

Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi.

N Biotechnol. 2018 Nov 25;46:1-6

Authors: Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T

Abstract

In the present study, we have characterized high molecular weight multi-enzyme complexes in two commercial enzymes produced by Trichoderma reesei (Spezyme CP) and Penicillium funiculosum (Accellerase XC). We successfully identified 146-1000?kDa complexes using Blue native polyacrylamide gel electrophoresis (BN-PAGE) to fractionate the protein profile in both preparations. Identified complexes dissociated into lower molecular weight constituents when loaded on SDS PAGE. Unfolding of the secondary structure of multi-enzyme complexes with trimethylamine (pH >10) suggested that they were not a result of unspecific protein aggregation. Cellulase (CMCase) profiles of extracts of BN-PAGE fractionated protein bands confirmed cellulase activity within the multi-enzyme complexes. A microassay was used to identify protein bands that promoted high levels of glucose release from barley straw. Those with high saccharification yield were subjected to LC-MS analysis to identify the principal enzymatic activities responsible. The results suggest that secretion of proteins by aerobic fungi leads to the formation of high molecular weight multi-enzyme complexes that display activity against carboxymethyl cellulose and barley straw.

PMID: 29803771 [PubMed - indexed for MEDLINE]





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