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PubMed Publications| Keyword search (4,163 papers available) |  |
"Greco BM" Authored Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | PARPAL: PARalog Protein Redistribution using Abundance and Localization in Yeast Database | Greco BM; Zapata G; Dandage R; Papkov M; Pereira V; Lefebvre F; Bourque G; Parts L; Kuzmin E; | 40580499 BIOLOGY |
| 2 | Single-cell imaging of protein dynamics of paralogs reveals sources of gene retention | Dandage R; Papkov M; Greco BM; Pereira V; Fishman D; Friesen H; Wang K; Styles EB; Kraus O; Grys B; Zapata G; Lefebvre F; Bourque G; Boone C; Andrews BJ; Parts L; Kuzmin E; | 40585364 BIOLOGY |
| 3 | A Humanized Yeast Model for Studying TRAPP Complex Mutations; Proof-of-Concept Using Variants from an Individual with a TRAPPC1-Associated Neurodevelopmental Syndrome | Zykaj E; Abboud C; Asadi P; Warsame S; Almousa H; Milev MP; Greco BM; López-Sánchez M; Bratkovic D; Kachroo AH; Pérez-Jurado LA; Sacher M; | 39273027 BIOLOGY |
| 4 | Single-cell imaging of protein dynamics of paralogs reveals mechanisms of gene retention | Dandage R; Papkov M; Greco BM; Fishman D; Friesen H; Wang K; Styles E; Kraus O; Grys B; Boone C; Andrews B; Parts L; Kuzmin E; | 38045359 BIOLOGY |
| 5 | Rapid, scalable, combinatorial genome engineering by marker-less enrichment and recombination of genetically engineered loci in yeast | Abdullah M; Greco BM; Laurent JM; Garge RK; Boutz DR; Vandeloo M; Marcotte EM; Kachroo AH; | 37323580 BIOLOGY |
| 6 | Humanized yeast to model human biology, disease and evolution | Kachroo AH; Vandeloo M; Greco BM; Abdullah M; | 35661208 BIOLOGY |
| Title: | Rapid, scalable, combinatorial genome engineering by marker-less enrichment and recombination of genetically engineered loci in yeast | ||||
| Authors: | Abdullah M, Greco BM, Laurent JM, Garge RK, Boutz DR, Vandeloo M, Marcotte EM, Kachroo AH | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/37323580/ | ||||
| DOI: | 10.1016/j.crmeth.2023.100464 | ||||
| Publication: | Cell reports methods | ||||
| Keywords: | CRISPR-Cas9; combinatorial ; genome editing; gene drive; humanized proteasome; humanized yeast; | ||||
| PMID: | 37323580 | Category: | Date Added: | 2023-06-16 | |
| Dept Affiliation: | BIOLOGY | ||||
Description: |
A major challenge to rationally building multi-gene processes in yeast arises due to the combinatorics of combining all of the individual edits into the same strain. Here, we present a precise and multi-site genome editing approach that combines all edits without selection markers using CRISPR-Cas9. We demonstrate a highly efficient gene drive that selectively eliminates specific loci by integrating CRISPR-Cas9-mediated double-strand break (DSB) generation and homology-directed recombination with yeast sexual assortment. The method enables marker-less enrichment and recombination of genetically engineered loci (MERGE). We show that MERGE converts single heterologous loci to homozygous loci at ~100% efficiency, independent of chromosomal location. Furthermore, MERGE is equally efficient at converting and combining multiple loci, thus identifying compatible genotypes. Finally, we establish MERGE proficiency by engineering a fungal carotenoid biosynthesis pathway and most of the human a-proteasome core into yeast. Therefore, MERGE lays the foundation for scalable, combinatorial genome editing in yeast. |
