Keyword search (4,163 papers available)

"Gold ND" Authored Publications:

Title Authors PubMed ID
1 Pathway elucidation and microbial synthesis of proaporphine and bis-benzylisoquinoline alkaloids from sacred lotus (Nelumbo nucifera) Pyne ME; Gold ND; Martin VJJ; 37004909
BIOLOGY
2 Author Correction: Building a global alliance of biofoundries. Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS 31296848
CHEMBIOCHEM
3 Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis. Gold ND, Martin VJ 17644599
BIOLOGY
4 Expression of a library of fungal β-glucosidases in Saccharomyces cerevisiae for the development of a biomass fermenting strain. Wilde C, Gold ND, Bawa N, Tambor JH, Mougharbel L, Storms R, Martin VJ 22218767
CSFG
5 Metabolic engineering of a tyrosine-overproducing yeast platform using targeted metabolomics. Gold ND, Gowen CM, Lussier FX, Cautha SC, Mahadevan R, Martin VJ 26016674
CSFG
6 Engineering of a Nepetalactol-Producing Platform Strain of Saccharomyces cerevisiae for the Production of Plant Seco-Iridoids. Campbell A, Bauchart P, Gold ND, Zhu Y, De Luca V, Martin VJ 26981892
CSFG
7 Reconstituting Plant Secondary Metabolism in Saccharomyces cerevisiae for Production of High-Value Benzylisoquinoline Alkaloids. Pyne ME, Narcross L, Fossati E, Bourgeois L, Burton E, Gold ND, Martin VJ 27417930
CSFG
8 Pyrenoid functions revealed by proteomics in Chlamydomonas reinhardtii Zhan Y; Marchand CH; Maes A; Mauries A; Sun Y; Dhaliwal JS; Uniacke J; Arragain S; Jiang H; Gold ND; Martin VJJ; Lemaire SD; Zerges W; 29481573
CSFG
9 A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast. Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ 30474973
CSFG
10 Building a global alliance of biofoundries. Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Le Feuvre R, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS 31068573
CHEMBIOCHEM

 

Title:A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.
Authors:Gold NDFossati EHansen CCDiFalco MDouchin VMartin VJJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/30474973?dopt=Abstract
DOI:10.1021/acssynbio.8b00470
Publication:ACS synthetic biology
Keywords:cytochrome P450 monooxygenasecytochrome P450 reductasediterpenoid biosynthesissteviol glucosides
PMID:30474973 Category:ACS Synth Biol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Centre for Applied Synthetic Biology , Concordia University , Montréal , Québec H4B 1R6 , Canada.
2 Plant Biochemistry Laboratory, Department of Plant and Environmental Science , University of Copenhagen , DK-1871 Frederiksberg C , Denmark.
3 Center for Synthetic Biology , University of Copenhagen , DK-1871 Frederiksberg C , Denmark.
4 Centre for Structural and Functional Genomics , Concordia University , Montréal , Québec H4B 1R6 , Canada.
5 Evolva , Lersø Parkallé 42-44 , DK-2100 Copenhagen Ø , Denmark.
6 Department of Biology, Centre for Structural and Functional Genomic , Concordia University , Montréal , Québec H4B 1R6 , Canada.

Description:

A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.

ACS Synth Biol. 2018 Dec 21;7(12):2918-2929

Authors: Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ

Abstract

Biosynthesis of steviol glycosides in planta proceeds via two cytochrome P450 enzymes (CYPs): kaurene oxidase (KO) and kaurenoic acid hydroxylase (KAH). KO and KAH function in succession with the support of a NADPH-dependent cytochrome P450 reductase (CPR) to convert kaurene to steviol. This work describes a platform for recombinant production of steviol glucosides (SGs) in Saccharomyces cerevisiae, demonstrating the full reconstituted pathway from the simple sugar glucose to the SG precursor steviol. With a focus on optimization of the KO-KAH activities, combinations of functional homologues were tested in batch growth. Among the CYPs, novel KO75 (CYP701) and novel KAH82 (CYP72) outperformed their respective functional homologues from Stevia rebaudiana, SrKO (CYP701A5) and SrKAH (CYP81), in assays where substrate was supplemented to culture broth. With kaurene produced from glucose in the cell, SrCPR1 from S. rebaudiana supported highest turnover for KO-KAH combinations, besting two other CPRs isolated from S. rebaudiana, the Arabidopsis thaliana ATR2, and a new class I CPR12. Some coexpressions of ATR2 with a second CPR were found to diminish KAH activity, showing that coexpression of CPRs can lead to competition for CYPs with possibly adverse effects on catalysis.

PMID: 30474973 [PubMed - in process]





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