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PubMed Publications| Keyword search (4,163 papers available) |  |
"Falco MD" Authored Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Genome and secretome insights: unravelling the lignocellulolytic potential of Myceliophthora verrucosa for enhanced hydrolysis of lignocellulosic biomass | Sharma G; Kaur B; Singh V; Raheja Y; Falco MD; Tsang A; Chadha BS; | 38676717 CSFG |
| 2 | Identification of a Conserved Transcriptional Activator-Repressor Module Controlling the Expression of Genes Involved in Tannic Acid Degradation and Gallic Acid Utilization in Aspergillus niger | Arentshorst M; Falco MD; Moisan MC; Reid ID; Spaapen TOM; van Dam J; Demirci E; Powlowski J; Punt PJ; Tsang A; Ram AFJ; | 37744122 CSFG |
| 3 | Secretion of small proteins is species-specific within Aspergillus sp. | Valette N, Benoit-Gelber I, Falco MD, Wiebenga A, de Vries RP, Gelhaye E, Morel-Rouhier M | 27153937 CSFG |
| Title: | Genome and secretome insights: unravelling the lignocellulolytic potential of Myceliophthora verrucosa for enhanced hydrolysis of lignocellulosic biomass | ||||
| Authors: | Sharma G, Kaur B, Singh V, Raheja Y, Falco MD, Tsang A, Chadha BS | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/38676717/ | ||||
| DOI: | 10.1007/s00203-024-03974-w | ||||
| Publication: | Archives of microbiology | ||||
| Keywords: | Myceliophthora verrucosa; CDH; Genome sequencing; Hydrolysis; LPMO; Secretome; | ||||
| PMID: | 38676717 | Category: | Date Added: | 2024-04-28 | |
| Dept Affiliation: |
CSFG
1 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. 2 Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6, Canada. 3 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. chadhabs@yahoo.com. |
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Description: |
Lignocellulolytic enzymes from a novel Myceliophthora verrucosa (5DR) strain was found to potentiate the efficacy of benchmark cellulase during saccharification of acid/alkali treated bagasse by ~ 2.24 fold, indicating it to be an important source of auxiliary enzymes. The De-novo sequencing and analysis of M. verrucosa genome (31.7 Mb) revealed to encode for 7989 putative genes, representing a wide array of CAZymes (366) with a high proportions of auxiliary activity (AA) genes (76). The LC/MS QTOF based secretome analysis of M. verrucosa showed high abundance of glycosyl hydrolases and AA proteins with cellobiose dehydrogenase (CDH) (AA8), being the most prominent auxiliary protein. A gene coding for lytic polysaccharide monooxygenase (LPMO) was expressed in Pichia pastoris and CDH produced by M. verrucosa culture on rice straw based solidified medium were purified and characterized. The mass spectrometry of LPMO catalyzed hydrolytic products of avicel showed the release of both C1/C4 oxidized products, indicating it to be type-3. The lignocellulolytic cocktail comprising of in-house cellulase produced by Aspergillus allahabadii strain spiked with LPMO & CDH exhibited enhanced and better hydrolysis of mild alkali deacetylated (MAD) and unwashed acid pretreated rice straw slurry (UWAP), when compared to Cellic CTec3 at high substrate loading rate. |
