Keyword search (4,163 papers available)

"Bourque SD" Authored Publications:

Title Authors PubMed ID
1 Metabolomic and lipidomic analyses of chronologically aging yeast. Richard VR, Bourque SD, Titorenko VI 25213255
BIOLOGY
2 Caloric restriction delays yeast chronological aging by remodeling carbohydrate and lipid metabolism, altering peroxisomal and mitochondrial functionalities, and postponing the onsets of apoptotic and liponecrotic modes of regulated cell death. Arlia-Ciommo A, Leonov A, Beach A, Richard VR, Bourque SD, Burstein MT, Kyryakov P, Gomez-Perez A, Koupaki O, Feldman R, Titorenko VI 29662634
BIOLOGY
3 Mechanisms through which lithocholic acid delays yeast chronological aging under caloric restriction conditions. Arlia-Ciommo A, Leonov A, Mohammad K, Beach A, Richard VR, Bourque SD, Burstein MT, Goldberg AA, Kyryakov P, Gomez-Perez A, Koupaki O, Titorenko VI 30405886
BIOLOGY

 

Title:Metabolomic and lipidomic analyses of chronologically aging yeast.
Authors:Richard VR,  Bourque SD,  Titorenko VI
Link:https://www.ncbi.nlm.nih.gov/pubmed/25213255?dopt=Abstract
DOI:10.1007/978-1-4939-1363-3_21
Publication:Methods in molecular biology (Clifton, N.J.)
Keywords:
PMID:25213255 Category:Methods Mol Biol Date Added:2019-06-20
Dept Affiliation: BIOLOGY
1 Department of Biology, Concordia University, 7141 Sherbrooke Street, West, SP Building, Montreal, QC, Canada, H4B 1R6.

Description:

Metabolomic and lipidomic analyses of chronologically aging yeast.

Methods Mol Biol. 2014;1205:359-73

Authors: Richard VR, Bourque SD, Titorenko VI

Abstract

Metabolomic and lipidomic analyses of yeast cells provide comprehensive empirical datasets for unveiling mechanisms underlying complex biological processes. In this chapter, we describe detailed protocols for using such analyses to study the age-related dynamics of changes in intracellular and extracellular levels of various metabolites and membrane lipids in chronologically aging yeast. The protocols for the following high-throughput analyses are described: (1) microanalytic biochemical assays for monitoring intracellular concentrations of trehalose and glycogen; (2) gas chromatographic quantitative assessment of extracellular concentrations of ethanol and acetic acid; and (3) mass spectrometric identification and quantitation of the entire complement of cellular lipids. These protocols are applicable to the exploration of the metabolic patterns associated not only with aging but also with many other vital processes in yeast. The described here methodology complements the powerful genetic approaches available for mechanistic studies of fundamental aspects of yeast biology.

PMID: 25213255 [PubMed - indexed for MEDLINE]




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