Keyword search (4,163 papers available)

"Benoit-Gelber I" Authored Publications:

Title Authors PubMed ID
1 Global survey of secondary metabolism in em Aspergillus niger /em via activation of specific transcription factors Semper C; Pham TTM; Ram S; Palys S; Evdokias G; Ouedraogo JP; Moisan MC; Geoffrion N; Reid I; Di Falco M; Bailey Z; Tsang A; Benoit-Gelber I; Savchenko A; 40852424
GENOMICS
2 Identification of a Novel Biosynthetic Gene Cluster in Aspergillus niger Using Comparative Genomics Evdokias G; Semper C; Mora-Ochomogo M; Di Falco M; Nguyen TTM; Savchenko A; Tsang A; Benoit-Gelber I; 34064722
BIOLOGY
3 Evolutionary adaptation of Aspergillus niger for increased ferulic acid tolerance. Lubbers RJM, Liwanag AJ, Peng M, Dilokpimol A, Benoit-Gelber I, de Vries RP 31674709
CSFG
4 Secretion of small proteins is species-specific within Aspergillus sp. Valette N, Benoit-Gelber I, Falco MD, Wiebenga A, de Vries RP, Gelhaye E, Morel-Rouhier M 27153937
CSFG
5 Expression-based clustering of CAZyme-encoding genes of Aspergillus niger. Gruben BS, Mäkelä MR, Kowalczyk JE, Zhou M, Benoit-Gelber I, De Vries RP 29169319
CSFG
6 Evolutionary Adaptation to Generate Mutants. de Vries RP, Lubbers R, Patyshakuliyeva A, Wiebenga A, Benoit-Gelber I 29876815
BIOLOGY
7 The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum. Coconi Linares N, Di Falco M, Benoit-Gelber I, Gruben BS, Peng M, Tsang A, Mäkelä MR, de Vries RP 30797054
CSFG

 

Title:Evolutionary adaptation of Aspergillus niger for increased ferulic acid tolerance.
Authors:Lubbers RJM,  Liwanag AJ,  Peng M,  Dilokpimol A,  Benoit-Gelber I,  de Vries RP
Link:https://www.ncbi.nlm.nih.gov/pubmed/31674709?dopt=Abstract
DOI:10.1111/jam.14505
Publication:Journal of applied microbiology
Keywords:Aspergillus;  adaptive evolution;  aromatic metabolism;  cell factory;  ferulic acid tolerance;  mutagenesis; 
PMID:31674709 Category:J Appl Microbiol Date Added:2019-11-02
Dept Affiliation: CSFG
1 Fungal Physiology, Westerdijk Fungal Biodiversity Institute, Fungal Molecular Physiology, Utrecht University, Utrecht, The Netherlands.
2 Centre for Structural and Functional Genomics, Concordia University, Montréal, Canada.

Description:

Evolutionary adaptation of Aspergillus niger for increased ferulic acid tolerance.

J Appl Microbiol. 2019 Nov 01;:

Authors: Lubbers RJM, Liwanag AJ, Peng M, Dilokpimol A, Benoit-Gelber I, de Vries RP

Abstract

AIMS: To create an Aspergillus niger mutant with increased tolerance against ferulic acid using evolutionary adaptation.

METHODS AND RESULTS: Evolutionary adaptation of Aspergillus niger N402 was performed by consecutive growth on increasing concentrations of ferulic acid in the presence of 25 mmol l-1 D-fructose, starting from 0·5 mmol l-1 and ending with 5 mmol l-1 ferulic acid. The A. niger mutant obtained after six months, named Fa6, showed increased ferulic acid tolerance compared to the parent. In addition, Fa6 has increased ferulic acid consumption and a higher conversion rate, suggesting that the mutation affects aromatic metabolism of this species. Transcriptome analysis of the evolutionary mutant on ferulic acid revealed a distinct gene expression profile compared to the wild type. Further analysis of this mutant and the parent strain provided the first experimental confirmation that A. niger converts coniferyl alcohol to ferulic acid.

CONCLUSIONS: The evolutionary adaptive A. niger mutant Fa6 has beneficial mutations that increase the tolerance, conversion rate and uptake of ferulic acid.

SIGNIFICANCE AND IMPACT OF THE STUDY: This study demonstrates that evolutionary adaptation is a powerful tool to modify microorganisms towards increased tolerance to harsh conditions, which is beneficial for various industrial applications.

PMID: 31674709 [PubMed - as supplied by publisher]




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