Keyword search (4,163 papers available)

"Bellemare A" Authored Publications:

Title Authors PubMed ID
1 Expansion of Auxiliary Activity Family 5 sequence space via biochemical characterization of six new copper radical oxidases Fong JK; Mathieu Y; Vo MT; Bellemare A; Tsang A; Brumer H; 38953370
CSFG
2 Functional screening pipeline to uncover laccase-like multicopper oxidase enzymes that transform industrial lignins Sharan AA; Bellemare A; DiFalco M; Tsang A; Vuong TV; Edwards EA; Master ER; 38000639
CSFG
3 Functional characterization of fungal lytic polysaccharide monooxygenases for cellulose surface oxidation Mathieu Y; Raji O; Bellemare A; Di Falco M; Nguyen TTM; Viborg AH; Tsang A; Master E; Brumer H; 37679837
CSFG
4 Characterization of a novel AA3_1 xylooligosaccharide dehydrogenase from Thermothelomyces myriococcoides CBS 398.93 Zhao H; Karppi J; Nguyen TTM; Bellemare A; Tsang A; Master E; Tenkanen M; 36476312
CSFG
5 Carbohydrate esterase family 16 contains fungal hemicellulose acetyl esterases (HAEs) with varying specificity Venegas FA; Koutaniemi S; Langeveld SMJ; Bellemare A; Chong SL; Dilokpimol A; Lowden MJ; Hilden KS; Leyva-Illades JF; Mäkelä MR; My Pham TT; Peng M; Hancock MA; Zheng Y; Tsang A; Tenkanen M; Powlowski J; de Vries RP; 35405333
CSFG
6 Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases Dilokpimol A; Verkerk B; Li X; Bellemare A; Lavallee M; Frommhagen M; Nørmølle Underlin E; Kabel MA; Powlowski J; Tsang A; de Vries RP; 35187647
CSFG
7 Fungal Genomic DNA Extraction Methods for Rapid Genotyping and Genome Sequencing. Bellemare A, John T, Marqueteau S 29876805
CSFG

 

Title:Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases
Authors:Dilokpimol AVerkerk BLi XBellemare ALavallee MFrommhagen MNørmølle Underlin EKabel MAPowlowski JTsang Ade Vries RP
Link:https://pubmed.ncbi.nlm.nih.gov/35187647/
DOI:10.1002/1873-3468.14322
Publication:FEBS letters
Keywords:acetyl xylan esterasecarbohydrate esterase family 1feruloyl esterasefungihydroxycinnamic acidplant biomass
PMID:35187647 Category: Date Added:2022-02-21
Dept Affiliation: CSFG
1 Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
2 Current address: Protein Production Team, VTT Technical Research Center of Finland Ltd, Tietotie 2, 02150, Espoo, Finland.
3 Centre for Functional and Structural Genomics, Concordia University, Sherbrooke St. W. Montreal, QC, H4B 1R6, Canada.
4 Laboratory of Food Chemistry, Wageningen University and Research, Bornse Weilanden 9, 6708 WG, Wageningen, The Netherlands.
5 Department of Chemistry, Technical University of Denmark, Building 207, Kemitorvet, DK-2800, Denmark.

Description:

Feruloyl esterases (FAEs) and acetyl xylan esterases (AXEs) are important enzymes for plant biomass degradation and are both present in Carbohydrate Esterase family 1 (CE1) of the Carbohydrate-Active enZymes database. In this study, ten novel fungal CE1 enzymes from different subfamilies were heterologously produced and screened for their activity towards model and complex plant biomass substrates. CE1_1 enzymes possess AXE activity, while CE1_5 enzymes showed FAE activity. Two enzymes from CE1_2 and one from CE1_5 possess dual feruloyl/acetyl xylan esterase (FXE) activity, showing expansion of substrate specificity. The new FXEs from CE1 can efficiently release both feruloyl and acetyl residues from feruloylated xylan, making them particularly interesting novel components of industrial enzyme cocktails for plant biomass degradation.





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