Keyword search (4,163 papers available)

"Arentshorst M" Authored Publications:

Title Authors PubMed ID
1 An examination of the quinic acid utilization genes in Aspergillus niger reveals the involvement of two pH-dependent permeases Sgro M; Reid ID; Arentshorst M; Ram AFJ; Tsang A; 40853219
GENOMICS
2 Identification of a Conserved Transcriptional Activator-Repressor Module Controlling the Expression of Genes Involved in Tannic Acid Degradation and Gallic Acid Utilization in Aspergillus niger Arentshorst M; Falco MD; Moisan MC; Reid ID; Spaapen TOM; van Dam J; Demirci E; Powlowski J; Punt PJ; Tsang A; Ram AFJ; 37744122
CSFG
3 Utilization of ferulic acid in Aspergillus niger requires the transcription factor FarA and a newly identified Far-like protein (FarD) that lacks the canonical Zn(II)2Cys6 domain Arentshorst M; Reijngoud J; van Tol DJC; Reid ID; Arendsen Y; Pel HJ; van Peij NNME; Visser J; Punt PJ; Tsang A; Ram AFJ; 37746181
CSFG
4 Functional analysis of the protocatechuate branch of the β-ketoadipate pathway in Aspergillus niger Sgro M; Chow N; Olyaei F; Arentshorst M; Geoffrion N; Ram AFJ; Powlowski J; Tsang A; 37399977
BIOLOGY
5 Loss of function of the carbon catabolite repressor CreA leads to low but inducer-independent expression from the feruloyl esterase B promoter in Aspergillus niger Reijngoud J; Arentshorst M; Ruijmbeek C; Reid I; Alazi ED; Punt PJ; Tsang A; Ram AFJ; 33738610
CSFG
6 Genetic Characterization of Mutations Related to Conidiophore Stalk Length Development in Aspergillus niger Laboratory Strain N402 Demirci E; Arentshorst M; Yilmaz B; Swinkels A; Reid ID; Visser J; Tsang A; Ram AFJ; 33959152
CSFG
7 Deletion of the Aspergillus niger Pro-Protein Processing Protease Gene kexB Results in a pH-Dependent Morphological Transition during Submerged Cultivations and Increases Cell Wall Chitin Content. van Leeuwe TM, Arentshorst M, Forn-Cuní G, Geoffrion N, Tsang A, Delvigne F, Meijer AH, Ram AFJ, Punt PJ 33276589
CSFG
8 A set of isogenic auxotrophic strains for constructing multiple gene deletion mutants and parasexual crossings in Aspergillus niger. Niu J, Arentshorst M, Seelinger F, Ram AF, Ouedraogo JP 27251039
CSFG
9 An Evolutionarily Conserved Transcriptional Activator-Repressor Module Controls Expression of Genes for D-Galacturonic Acid Utilization in Aspergillus niger. Niu J, Alazi E, Reid ID, Arentshorst M, Punt PJ, Visser J, Tsang A, Ram AF 28049705
CSFG
10 The pathway intermediate 2-keto-3-deoxy-L-galactonate mediates the induction of genes involved in D-galacturonic acid utilization in Aspergillus niger. Alazi E, Khosravi C, Homan TG, du Pré S, Arentshorst M, Di Falco M, Pham TTM, Peng M, Aguilar-Pontes MV, Visser J, Tsang A, de Vries RP, Ram AFJ 28417461
CSFG
11 W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger. Alazi E, Niu J, Otto SB, Arentshorst M, Pham TTM, Tsang A, Ram AFJ 30298571
CSFG

 

Title:A set of isogenic auxotrophic strains for constructing multiple gene deletion mutants and parasexual crossings in Aspergillus niger.
Authors:Niu J,  Arentshorst M,  Seelinger F,  Ram AF,  Ouedraogo JP
Link:https://www.ncbi.nlm.nih.gov/pubmed/27251039?dopt=Abstract
DOI:10.1007/s00203-016-1240-6
Publication:Archives of microbiology
Keywords:Auxotrophy;  Isogenic strains;  Multiple markers;  Parasexual crossing; 
PMID:27251039 Category:Arch Microbiol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, Sylviusweg 72, 2333 BE, Leiden, The Netherlands.
2 Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, Sylviusweg 72, 2333 BE, Leiden, The Netherlands. a.f.j.ram@biology.leidenuniv.nl.
3 Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke St. W., Montreal, QC, H4B 1R6, Canada.

Description:

A set of isogenic auxotrophic strains for constructing multiple gene deletion mutants and parasexual crossings in Aspergillus niger.

Arch Microbiol. 2016 Nov;198(9):861-8

Authors: Niu J, Arentshorst M, Seelinger F, Ram AF, Ouedraogo JP

Abstract

To construct a set of isogenic auxotrophic strains in Aspergillus niger suited for creating multiple gene deletion mutants and executing parasexual crossings, we have combined mutations in genes involved in colour pigmentation (fwnA and olvA) with well-selectable auxotrophic markers (pyrG, nicB, argB, and adeA). All markers, except for the pyrG marker, were introduced by targeted deletion, omitting UV mutagenesis of the strains. Aspergillus oryzae orthologous genes of the argB, nicB, and adeA markers were used as heterologous selection markers, and all markers were shown to complement to respective auxotrophic A. niger mutants. A quadruple auxotrophic marker was further constructed suitable for multiple gene deletions. Genome sequencing of two auxotrophic colour mutants JN3.2 (olvA::pyrG, argB::hygB) and JN6.2 (olvA::pyrG, nicB::hygB) revealed four SNPs between them in non-coding regions, indicating a high level of isogenicity between both strains. The availability of near-isogenic complementary auxotrophic colour mutants facilitates the selection of diploids and the isolation of haploid segregants from the diploid using the parasexual cycle.

PMID: 27251039 [PubMed - indexed for MEDLINE]




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