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Evidence of isochorismate channeling between the Escherichia coli enterobactin biosynthetic enzymes EntC and EntB

Author(s): Bin X; Pawelek PD;

Enterobactin is a high-affinity iron chelator produced and secreted by Escherichia coli and Salmonella typhimurium to scavenge scarce extracellular Fe3+ as a micronutrient. EntC and EntB are the first two enzymes in the enterobactin biosynthetic pathway. Isochorismate, produced by EntC, is a substrate for EntB isochorismatase. By using a competing isochor ...

Article GUID: 39031458


Evidence of an intracellular interaction between the Escherichia coli enzymes EntC and EntB and identification of a potential electrostatic channeling surface

Author(s): Ouellette S; Pakarian P; Bin X; Pawelek PD;

Siderophores are high-affinity small-molecule chelators employed by bacteria to acquire iron from the extracellular environment. The Gram-negative bacterium Escherichia coli synthesizes and secretes enterobactin, a tris-catechol siderophore. Enterobactin is synthesized by six cytoplasmic enzyme activities: EntC, EntB (isochorismatase (IC) domain), EntA, E ...

Article GUID: 35952947


The stress induced caleosin, RD20/CLO3, acts as a negative regulator of GPA1 in Arabidopsis

Author(s): Brunetti SC; Arseneault MKM; Wright JA; Wang Z; Ehdaeivand MR; Lowden MJ; Rivoal J; Khalil HB; Garg G; Gulick PJ;

A stress induced calcium-binding protein, RD20/CLO3 interacts with the alpha subunit of the heterotrimeric G-protein complex in Arabidopsis and affects etiolation and leaf morphology. Heterotrimeric G proteins and calcium signaling have both been shown to play a role in the response to environmen ...

Article GUID: 34599731


Inter-protein residue covariation information unravels physically interacting protein dimers

Author(s): Salmanian S; Pezeshk H; Sadeghi M;

Background: Predicting physical interaction between proteins is one of the greatest challenges in computational biology. There are considerable various protein interactions and a huge number of protein sequences and synthetic peptides with unknown interacting counterparts. Most of co-evolutionary methods discover a combination of physical interplays and f ...

Article GUID: 33334319


Subunit orientation in the Escherichia coli enterobactin biosynthetic EntA-EntE complex revealed by a two-hybrid approach.

Author(s): Pakarian P, Pawelek PD

Biochimie. 2016 Aug;127:1-9 Authors: Pakarian P, Pawelek PD

Article GUID: 27086082


The evolutionary rewiring of the ribosomal protein transcription pathway modifies the interaction of transcription factor heteromer Ifh1-Fhl1 (interacts with forkhead 1-forkhead-like 1) with the DNA-binding specificity element.

Author(s): Mallick J, Whiteway M

J Biol Chem. 2013 Jun 14;288(24):17508-19 Authors: Mallick J, Whiteway M

Article GUID: 23625919


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