Author(s): Pontarelli A; Liu JT; Oh JK; Wilds CJ;

The protocols described in this article provide details regarding the synthesis and characterization of a disulfide containing linker phosphoramidite for terminal functionalization of synthetic oligonucleotides. The linker is first synthesized from 6-mercaptohexanol in two steps and is incorporated at the 5' end of short DNA oligonucleotides using aut ...

Article GUID: 36840706