Author(s): Jager K; Orozco-Hidalgo MT; Springstein BL; Joly-Smith E; Papazotos F; McDonough E; Fleming E; McCallum G; Yuan AH; Hilfinger A; Hochschild A; Potvin-Trottier L;

Prions are self-propagating protein aggregates formed by specific proteins that can adopt alternative folds. Prions were discovered as the cause of the fatal transmissible spongiform encephalopathies in mammals, but prions can also constitute nontoxic protein-based elements of inheritance in fung ...

Article GUID: 37738299

Author(s): Keramatnejad M; DeWolf C;

Ozone exposure from environmental smog has been implicated as a risk factor for developing dry eye disease (DED). The tear film lipid layer (TFLL), which is the outermost layer of the tear film and responsible for surface tension reduction while blinking, is in direct contact with the environment and serves as the first line of defense against external ag ...

Article GUID: 36837668

Author(s): Keramatnejad M; DeWolf C;

Tear film lipid layer (TFLL), the final layer of the human tear film is responsible for surface tension reduction while blinking, water evaporation retardation and maintaining the stability of the tear film. The study of the composition-structure-function relationship of TFLL is paramount, as a compromised structure of TFLL leads to the emergence of dry e ...

Article GUID: 36535341

Author(s): Husser MC; Ozugergin I; Resta T; Martin VJJ; Piekny AJ;

Cytokinesis is required to physically separate the daughter cells at the end of mitosis. This crucial process requires the assembly and ingression of an actomyosin ring, which must occur with high fidelity to avoid aneuploidy and cell fate changes. Most of our knowledge of mammalian cytokinesis was generated using over-expressed transgenes in HeLa cells. ...

Article GUID: 36416720

Author(s): Allard P; Papazotos F; Potvin-Trottier L;

Cells are inherently dynamic, whether they are responding to environmental conditions or simply at equilibrium, with biomolecules constantly being made and destroyed. Due to their small volumes, the chemical reactions inside cells are stochastic, such that genetically identical cells display heterogeneous behaviors and gene expression profiles. Studying t ...

Article GUID: 36312536

Author(s): Bettauer V; Costa ACBP; Omran RP; Massahi S; Kirbizakis E; Simpson S; Dumeaux V; Law C; Whiteway M; Hallett MT;

We present deep learning-based approaches for exploring the complex array of morphologies exhibited by the opportunistic human pathogen Candida albicans. Our system, entitled Candescence, automatically detects C. albicans cells from differential image contrast microscopy and labels each detected ...

Article GUID: 35972285

Author(s): Sohrabi Kashani A; Larocque K; Piekny A; Packirisamy M;

In the present study, we investigate the mechanobiological responses of human lung cancer that may occur through their interactions with two different types of gold nanoparticles: nanostars and nanospheres. Hyperspectral images of nanoparticle-treated cells revealed different spatial distributions of nanoparticles in cells depending on their morphology, w ...

Article GUID: 35839330

Author(s): Bean BDM; Whiteway M; Martin VJJ;

The genetic tractability of the yeast Saccharomyces cerevisiae has made it a key model organism for basic research and a target for metabolic engineering. To streamline the introduction of tagged genes and compartmental markers with powerful CRISPR-Cas9-based genome editing tools we constructed a Markerless Yeast Localization and Overexpression (MyLO) CRI ...

Article GUID: 35708612

Author(s): Almey A; Milner TA; Brake WG;

Estrogens affect dopamine-dependent diseases/behavior and have rapid effects on dopamine release and receptor availability in the nucleus accumbens (NAc). Low levels of nuclear estrogen receptor (ER) a and ERß are seen in the NAc, which cannot account for the rapid effects of estrogens in this region. G-protein coupled ER 1 (GPER1) is observed at low leve ...

Article GUID: 35397175

Author(s): MacKeigan PW; Garner RE; Monchamp MÈ; Walsh DA; Onana VE; Kraemer SA; Pick FR; Beisner BE; Agbeti MD; da Costa NB; Shapiro BJ; Gregory-Eaves I;

Accurately identifying the species present in an ecosystem is vital to lake managers and successful bioassessment programs. This is particularly important when monitoring cyanobacteria, as numerous taxa produce toxins and can have major negative impacts on aquatic ecosystems. Increasingly, DNA-ba ...

Article GUID: 35287928

Author(s): Rahman A, Zunair H, Reme TR, Rahman MS, Mahdy MRC

Malaria, one of the leading causes of death in underdeveloped countries, is primarily diagnosed using microscopy. Computer-aided diagnosis of malaria is a challenging task owing to the fine-grained variability in the appearance of some uninfected and infected class. In this paper, we transform a malaria parasite object detection dataset into a classificat ...

Article GUID: 33465520