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Tri-Functional CRISPR Screen Reveals Overexpression of em QDR2 /em and em QDR3 /em Transporters Increase Fumaric Acid Production in em Kluyveromyces marxianus /em

Author(s): Thornbury M; Omran RP; Kumar L; Knoops A; Abushahin R; Whiteway M; Martin VJJ;

Organic acids such as fumaric acid are widely used in the food and beverage industry as acidulants and preservatives, while also serving as versatile precursors for industrially relevant compounds. Fumaric acid is still predominantly produced through petroleum-derived processes. To enhance production efficiency and diversify supply, we are engineering Klu ...

Article GUID: 41277095


Advanced lithium-ion battery process manufacturing equipment for gigafactories: Past, present, and future perspectives

Author(s): Nekahi A; Feyzi E; Srivastava M; Yeganehdoust F; Madikere Raghunagtha Reddy AK; Zaghib K;

Lithium-ion battery cell manufacturing depends on a few key raw materials and equipment manufacturers. Battery manufacturing faces global challenges and opportunities as various regions, including Asia, Europe, North America, and emerging markets, seek to scale gigafactory production and innovate equipment manufacturing pathways. Regions can enhance batte ...

Article GUID: 40678540


Sequencing of a Dairy Isolate Unlocks em Kluyveromyces marxianus /em as a Host for Lactose Valorization

Author(s): Thornbury M; Knoops A; Summerby-Murray I; Dhaliwal J; Johnson S; Utomo JC; Joshi J; Narcross L; Remondetto G; Pouliot M; Whiteway M; Martin VJJ;

The use of genetically modified nonconventional yeast provides significant potential for the bioeconomy by diversifying the tools available for the development of sustainable and novel products. In this study, we sequenced and annotated the genome of Kluyveromyces marxianus Y-1190 to establish it ...

Article GUID: 40629255


Exploring the glycoprotein washing fluid-assisted cleanup for the restoration of oil-contaminated shorelines with environmental integrity

Author(s): Sui J; Yue R; Bi H; Fu H; Yang A; Wang M; An C;

Spilled oil in ocean can spread to the shoreline and cause long-term impacts on the shoreline's ecological environment. Therefore, removing oil accumulated on shorelines is crucial. This study proposed an innovative ovalbumin (OVA) fluid-assisted method for the cleanup of oiled shoreline substrates. The oil removal efficiency of OVA fluids was systema ...

Article GUID: 39260515


Advancements in Hybrid Cellulose-Based Films: Innovations and Applications in 2D Nano-Delivery Systems

Author(s): Ramezani G; Stiharu I; van de Ven TGM; Nerguizian V;

This review paper delves into the realm of hybrid cellulose-based materials and their applications in 2D nano-delivery systems. Cellulose, recognized for its biocompatibility, versatility, and renewability, serves as the core matrix for these nanomaterials. The paper offers a comprehensive overview of the latest advancements in the creation, analysis, and ...

Article GUID: 38667550


ALBA: a model-driven framework for the automatic generation of android location-based apps

Author(s): Gharaat M; Sharbaf M; Zamani B; Hamou-Lhadj A;

In recent years, the number of smartphone users has increased dramatically. These users download millions of apps and use them for various services. Due to the significant demand for mobile apps, developers often seek faster development methods and more effective tools and techniques to generate these apps. Many of these apps are location-based apps in wh ...

Article GUID: 38624616


The effect of Wharton's jelly-derived stem cells seeded/boron-loaded acellular scaffolds on the healing of full-thickness burn wounds in the rat model

Author(s): Nikzad S; Same S; Safiri S; Dolati S; Roushangar Zineh B; Meshgi S; Roshangar L; Sahin F;

Burn wounds are the most destructive and complicated type of skin or underlying soft tissue injury that are exacerbated by a prolonged inflammatory response. Several cell-based therapeutic systems through the culturing of potent stem cells on modified scaffolds have been developed to direct the burn healing challenges. In this context, a new regenerative ...

Article GUID: 38364284


Multi-objective control optimization of isolated bridge using replicator controller and NSGA-II

Author(s): Momeni Z; Bagchi A;

Earthquakes can cause significant damage to constructed structures, leading engineers to design systems that effectively reduce damage and improve real-time vibration control. While base isolation is a commonly used passive method for seismic protection in highway structures, it has limitations such as a lack of immediate adaptation, constrained power dis ...

Article GUID: 37662735


CRAPS: Chromosomal-Repair-Assisted Pathway Shuffling in Yeast

Author(s): Dykstra CB; Pyne ME; Martin VJJ;

A fundamental challenge of metabolic engineering involves assembling and screening vast combinations of orthologous enzymes across a multistep biochemical pathway. Current pathway assembly workflows involve combining genetic parts ex vivo and assembling one pathway configuration per tube or well. Here, we present CRAPS, Chromosomal-Repair-Assisted Pathway ...

Article GUID: 37584634


Facilitation strength across environmental and beneficiary trait gradients in stream communities

Author(s): Tumolo BB; Albertson LK; Daniels MD; Cross WF; Sklar LL;

Ecosystem engineers modify habitats in ways that facilitate other community members by ameliorating harsh conditions. The strength of such facilitation is predicted to be influenced by both beneficiary traits and abiotic context. One key trait of animals that could control the strength of facilitation is beneficiary body size because it should determine h ...

Article GUID: 37555442


Functional Synthetic Biology

Author(s): Aldulijan I; Beal J; Billerbeck S; Bouffard J; Chambonnier G; Ntelkis N; Guerreiro I; Holub M; Ross P; Selvarajah V; Sprent N; Vidal G; Vignoni A;

Synthetic biologists have made great progress over the past decade in developing methods for modular assembly of genetic sequences and in engineering biological systems with a wide variety of functions in various contexts and organisms. However, current paradigms in the field entangle sequence an ...

Article GUID: 37073284


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