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Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies

Author(s): Husser MC; Pham NP; Law C; Araujo FRB; Martin VJJ; Piekny A;

Endogenous tags have become invaluable tools to visualize and study native proteins in live cells. However, generating human cell lines carrying endogenous tags is difficult due to the low efficiency of homology-directed repair. Recently, an engineered split mNeonGreen protein was used to generate a large-scale endogenous tag library in HEK293 cells. Usin ...

Article GUID: 38652106


Cancer anorexia-cachexia syndrome is characterized by more than one inflammatory pathway

Author(s): Bruno Gagnon

CONCLUSIONS: This exploratory study identified three distinct pathways of inflammation, or lack thereof, characterizing different CACS phenotypes.

Article GUID: 38481033


Diversity is the spice of life: An overview of how cytokinesis regulation varies with cell type

Author(s): Ozugergin I; Piekny A;

Cytokinesis is required to physically cleave a cell into two daughters at the end of mitosis. Decades of research have led to a comprehensive understanding of the core cytokinesis machinery and how it is regulated in animal cells, however this knowledge was generated using single cells cultured in vitro, or in early embryos before tissues develop. This ra ...

Article GUID: 36420142


Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA

Author(s): Husser MC; Ozugergin I; Resta T; Martin VJJ; Piekny AJ;

Cytokinesis is required to physically separate the daughter cells at the end of mitosis. This crucial process requires the assembly and ingression of an actomyosin ring, which must occur with high fidelity to avoid aneuploidy and cell fate changes. Most of our knowledge of mammalian cytokinesis was generated using over-expressed transgenes in HeLa cells. ...

Article GUID: 36416720


Diverse mechanisms regulate contractile ring assembly for cytokinesis in the two-cell C. elegans embryo

Author(s): Ozugergin I; Mastronardi K; Law C; Piekny A;

Cytokinesis occurs at the end of mitosis due to the ingression of a contractile ring that cleaves the daughter cells. The core machinery regulating this crucial process is conserved among metazoans. Multiple pathways control ring assembly, but their contribution in different cell types is not known. We found that in the C. elegans embryo, AB and P1 cells ...

Article GUID: 35022791


Seeing is believing: tools to study the role of Rho GTPases during cytokinesis

Author(s): Koh SP; Pham NP; Piekny A;

Cytokinesis is required to cleave the daughter cells at the end of mitosis and relies on the spatiotemporal control of RhoA GTPase. Cytokinesis failure can lead to changes in cell fate or aneuploidy, which can be detrimental during development and/or can lead to cancer. However, our knowledge of the pathways that regulate RhoA during cytokinesis is limite ...

Article GUID: 34405757


Complementary functions for the Ran gradient during division.

Author(s): Ozugergin I, Piekny A

Small GTPases. 2020 Feb 03;: Authors: Ozugergin I, Piekny A

Article GUID: 32013678


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