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Evidence of isochorismate channeling between the Escherichia coli enterobactin biosynthetic enzymes EntC and EntB

Author(s): Bin X; Pawelek PD;

Enterobactin is a high-affinity iron chelator produced and secreted by Escherichia coli and Salmonella typhimurium to scavenge scarce extracellular Fe3+ as a micronutrient. EntC and EntB are the first two enzymes in the enterobactin biosynthetic pathway. Isochorismate, produced by EntC, is a substrate for EntB isochorismatase. By using a competing isochor ...

Article GUID: 39031458


Evidence of an intracellular interaction between the Escherichia coli enzymes EntC and EntB and identification of a potential electrostatic channeling surface

Author(s): Ouellette S; Pakarian P; Bin X; Pawelek PD;

Siderophores are high-affinity small-molecule chelators employed by bacteria to acquire iron from the extracellular environment. The Gram-negative bacterium Escherichia coli synthesizes and secretes enterobactin, a tris-catechol siderophore. Enterobactin is synthesized by six cytoplasmic enzyme activities: EntC, EntB (isochorismatase (IC) domain), EntA, E ...

Article GUID: 35952947


Loss of Arp1, a putative actin-related protein, triggers filamentous and invasive growth and impairs pathogenicity in Candida albicans.

Author(s): Yao S, Feng Y, Islam A, Shrivastava M, Gu H, Lu Y, Sheng J, Whiteway M, Feng J

The polymorphous cellular shape of Candida albicans, in particular the transition from a yeast to a filamentous form, is crucial for either commensalism or life-threatening infections of the host. Various external or internal stimuli, including serum and nutrition starvation, have been shown to regulate filamentous growth primarily through two classical s ...

Article GUID: 33363697


Transendothelial Perforations and the Sphere of Influence of Single-Site Sonoporation.

Author(s): Helfield B, Chen X, Watkins SC, Villanueva FS

Ultrasound Med Biol. 2020 May 10;: Authors: Helfield B, Chen X, Watkins SC, Villanueva FS

Article GUID: 32402675


Subunit orientation in the Escherichia coli enterobactin biosynthetic EntA-EntE complex revealed by a two-hybrid approach.

Author(s): Pakarian P, Pawelek PD

Biochimie. 2016 Aug;127:1-9 Authors: Pakarian P, Pawelek PD

Article GUID: 27086082


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