Author(s): Arentshorst M; Reijngoud J; van Tol DJC; Reid ID; Arendsen Y; Pel HJ; van Peij NNME; Visser J; Punt PJ; Tsang A; Ram AFJ;

The feruloyl esterase B gene (faeB) is specifically induced by hydroxycinnamic acids (e.g. ferulic acid, caffeic acid and coumaric acid) but the transcriptional regulation network involved in faeB induction and ferulic acid metabolism has only been partially addressed. To identify transcription f ...

Article GUID: 37746181

Author(s): Reijngoud J; Arentshorst M; Ruijmbeek C; Reid I; Alazi ED; Punt PJ; Tsang A; Ram AFJ;

Objective: With the aim to decipher the mechanisms involved in the transcriptional regulation of feruloyl esterase encoded by faeB, a genetic screen was performed to isolate A. niger mutants displaying inducer-independent expression from the faeB promoter. Result: PfaeB-amdS and PfaeB-lux dual reporter strains were constructed and used to isolate trans-a ...

Article GUID: 33738610