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An electrochemical aptasensor for Δ9-tetrahydrocannabinol detection in saliva on a microfluidic platform

Author(s): Kékedy-Nagy L; Perry JM; Little SR; Llorens OY; Shih SCC;

We present a novel "on-off", cost-effective, rapid electrochemical aptasensor combined with a microfluidics cartridge system for the detection of ?9-THC (?9-tetrahydrocannabinol) in human saliva via differential pulse voltammetry. The assay relied on the competitive binding between the ?9-THC and a soluble redox indicator methylene blue, using an aptamer ...

Article GUID: 36549107


A Synthetic Biosensor for Detecting Putrescine in Beef Samples

Author(s): Selim AS; Perry JM; Nasr MA; Pimprikar JM; Shih SCC;

Biogenic amines (BAs) are toxicological risks present in many food products. Putrescine is the most common foodborne BA and is frequently used as a quality control marker. Currently, there is a lack of regulation concerning safe putrescine limits in food as well as outdated food handling practices leading to unnecessary putrescine intake. Conventional met ...

Article GUID: 36356104


Expanding the limits towards 'one-pot' DNA assembly and transformation on a rapid-prototype microfluidic device

Author(s): Perry JM; Soffer G; Jain R; Shih SCC;

DNA assembly and transformation are crucial to the building process in synthetic biology. These steps are significant roadblocks when engineering increasingly complex biological systems. To address this, recent development of widespread 'biofoundry' facilities has employed automation equipment to expedite the synthetic biology workflow. Despite si ...

Article GUID: 34369550


Real-Time Optogenetics System for Controlling Gene Expression Using a Model-Based Design.

Author(s): Soffer G, Perry JM, Shih SCC

Optimization of engineered biological systems requires precise control over the rates and timing of gene expression. Optogenetics is used to dynamically control gene expression as an alternative to conventional chemical-based methods since it provides a more convenient interface between digital control software and microbial culture. Here, we describe the ...

Article GUID: 33543619


Integration of World-to-Chip Interfaces with Digital Microfluidics for Bacterial Transformation and Enzymatic Assays.

Author(s): Moazami E, Perry JM, Soffer G, Husser MC, Shih SCC

Anal Chem. 2019 Apr 16;91(8):5159-5168 Authors: Moazami E, Perry JM, Soffer G, Husser MC, Shih SCC

Article GUID: 30945840


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