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Evidence of isochorismate channeling between the Escherichia coli enterobactin biosynthetic enzymes EntC and EntB

Author(s): Bin X; Pawelek PD;

Enterobactin is a high-affinity iron chelator produced and secreted by Escherichia coli and Salmonella typhimurium to scavenge scarce extracellular Fe3+ as a micronutrient. EntC and EntB are the first two enzymes in the enterobactin biosynthetic pathway. Isochorismate, produced by EntC, is a substrate for EntB isochorismatase. By using a competing isochor ...

Article GUID: 39031458


Evidence of an intracellular interaction between the Escherichia coli enzymes EntC and EntB and identification of a potential electrostatic channeling surface

Author(s): Ouellette S; Pakarian P; Bin X; Pawelek PD;

Siderophores are high-affinity small-molecule chelators employed by bacteria to acquire iron from the extracellular environment. The Gram-negative bacterium Escherichia coli synthesizes and secretes enterobactin, a tris-catechol siderophore. Enterobactin is synthesized by six cytoplasmic enzyme activities: EntC, EntB (isochorismatase (IC) domain), EntA, E ...

Article GUID: 35952947


A shared binding site for NAD+ and coenzyme A in an acetaldehyde dehydrogenase involved in bacterial degradation of aromatic compounds.

Author(s): Lei Y, Pawelek PD, Powlowski J

Biochemistry. 2008 Jul 01;47(26):6870-82 Authors: Lei Y, Pawelek PD, Powlowski J

Article GUID: 18537268


Subunit orientation in the Escherichia coli enterobactin biosynthetic EntA-EntE complex revealed by a two-hybrid approach.

Author(s): Pakarian P, Pawelek PD

Biochimie. 2016 Aug;127:1-9 Authors: Pakarian P, Pawelek PD

Article GUID: 27086082


Intracellular co-localization of the Escherichia coli enterobactin biosynthetic enzymes EntA, EntB, and EntE.

Author(s): Pakarian P, Pawelek PD

Biochem Biophys Res Commun. 2016 09 09;478(1):25-32 Authors: Pakarian P, Pawelek PD

Article GUID: 27470582


A novel set of vectors for Fur-controlled protein expression under iron deprivation in Escherichia coli.

Author(s): Pakarian P, Pawelek PD

BMC Biotechnol. 2016 09 13;16(1):68 Authors: Pakarian P, Pawelek PD

Article GUID: 27619907


Dual Activity of Rose Bengal Functionalized to Albumin-Coated Lanthanide-Doped Upconverting Nanoparticles: Targeting and Photodynamic Therapy.

Author(s): Sabri T, Pawelek PD, Capobianco JA

ACS Appl Mater Interfaces. 2018 Aug 15;10(32):26947-26953 Authors: Sabri T, Pawelek PD, Capobianco JA

Article GUID: 30028124


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