Reset filters

Search publications


Search by keyword
List by department / centre / faculty

No publications found.

 

A Humanized Yeast Model for Studying TRAPP Complex Mutations; Proof-of-Concept Using Variants from an Individual with a TRAPPC1-Associated Neurodevelopmental Syndrome

Author(s): Zykaj E; Abboud C; Asadi P; Warsame S; Almousa H; Milev MP; Greco BM; López-Sánchez M; Bratkovic D; Kachroo AH; Pérez-Jurado LA; Sacher M;

Variants in membrane trafficking proteins are known to cause rare disorders with severe symptoms. The highly conserved transport protein particle (TRAPP) complexes are key membrane trafficking regulators that are also involved in autophagy. Pathogenic genetic variants in specific TRAPP subunits a ...

Article GUID: 39273027


Erratum: Correction Notice: Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9

Author(s): Akhmetov A; Laurent JM; Gollihar J; Gardner EC; Garge RK; Ellington AD; Kachroo AH; Marcotte EM;

[This corrects the article .].

Article GUID: 38161732


Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeast

Author(s): Sultana S; Abdullah M; Li J; Hochstrasser M; Kachroo AH;

Yeast and humans share thousands of genes despite a billion years of evolutionary divergence. While many human genes can functionally replace their yeast counterparts, nearly half of the tested shared genes cannot. For example, most yeast proteasome subunits are "humanizable", except subunits comprising the ß-ring core, including ß2c (HsPSMB7, a constitut ...

Article GUID: 37364278


Rapid, scalable, combinatorial genome engineering by marker-less enrichment and recombination of genetically engineered loci in yeast

Author(s): Abdullah M; Greco BM; Laurent JM; Garge RK; Boutz DR; Vandeloo M; Marcotte EM; Kachroo AH;

A major challenge to rationally building multi-gene processes in yeast arises due to the combinatorics of combining all of the individual edits into the same strain. Here, we present a precise and multi-site genome editing approach that combines all edits without selection markers using CRISPR-Ca ...

Article GUID: 37323580


Humanized yeast to model human biology, disease and evolution

Author(s): Kachroo AH; Vandeloo M; Greco BM; Abdullah M;

For decades, budding yeast, a single-cellular eukaryote, has provided remarkable insights into human biology. Yeast and humans share several thousand genes despite morphological and cellular differences and over a billion years of separate evolution. These genes encode critical cellular processes, the failure of which in humans results in disease. Althoug ...

Article GUID: 35661208


Discovery of new vascular disrupting agents based on evolutionarily conserved drug action, pesticide resistance mutations, and humanized yeast

Author(s): Garge RK; Cha HJ; Lee C; Gollihar JD; Kachroo AH; Wallingford JB; Marcotte EM;

Thiabendazole (TBZ) is an FDA-approved benzimidazole widely used for its antifungal and antihelminthic properties. We showed previously that TBZ is also a potent vascular disrupting agent and inhibits angiogenesis at the tissue level by dissociating vascular endothelial cells in newly formed blood vessels. Here, we uncover TBZ's molecular target and m ...

Article GUID: 34849907


Humanization of yeast genes with multiple human orthologs reveals functional divergence between paralogs.

Author(s): Laurent JM, Garge RK, Teufel AI, Wilke CO, Kachroo AH, Marcotte EM

PLoS Biol. 2020 May 18;18(5):e3000627 Authors: Laurent JM, Garge RK, Teufel AI, Wilke CO, Kachroo AH, Marcotte EM

Article GUID: 32421706


Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9.

Author(s): Akhmetov A, Laurent JM, Gollihar J, Gardner EC, Garge RK, Ellington AD, Kachroo AH, Marcotte EM

Bio Protoc. 2018 Mar 20;8(6): Authors: Akhmetov A, Laurent JM, Gollihar J, Gardner EC, Garge RK, Ellington AD, Kachroo AH, Marcotte EM

Article GUID: 29770349


The Many Nuanced Evolutionary Consequences of Duplicated Genes.

Author(s): Teufel AI, Johnson MM, Laurent JM, Kachroo AH, Marcotte EM, Wilke CO

Mol Biol Evol. 2019 02 01;36(2):304-314 Authors: Teufel AI, Johnson MM, Laurent JM, Kachroo AH, Marcotte EM, Wilke CO

Article GUID: 30428072


-   Page 1 / 1   -