Author(s): Dumeaux V; Massahi S; Bettauer V; Mottola A; Dukovny A; Khurdia SS; Costa ACBP; Omran RP; Simpson S; Xie JL; Whiteway M; Berman J; Hallett MT;

Candida albicans, an opportunistic human pathogen, poses a significant threat to human health and is associated with significant socio-economic burden. Current antifungal treatments fail, at least in part, because C. albicans can initiate a strong drug tolerance response that allows some cells to ...

Article GUID: 37888959

Author(s): Simpson S; Bettauer V; Ramachandran A; Kraemer S; Mahon S; Medina M; Vallès Y; Dumeaux V; Vallès H; Walsh D; Hallett MT;

We study the microbiome of sea water collected from two locations of the Barbadian coral reefs. The two sites differ in several environmental and ecological variables including their endogenous benthic community and their proximity to urban development and runoffs from inland watersheds. The comp ...

Article GUID: 37009568

Author(s): Bettauer V; Costa ACBP; Omran RP; Massahi S; Kirbizakis E; Simpson S; Dumeaux V; Law C; Whiteway M; Hallett MT;

We present deep learning-based approaches for exploring the complex array of morphologies exhibited by the opportunistic human pathogen Candida albicans. Our system, entitled Candescence, automatically detects C. albicans cells from differential image contrast microscopy and labels each detected ...

Article GUID: 35972285

Author(s): Omran RP; Ramírez-Zavala B; Aji Tebung W; Yao S; Feng J; Law C; Dumeaux V; Morschhäuser J; Whiteway M;

Zinc cluster transcription factors are essential fungal regulators of gene expression. In the pathogen Candida albicans, the gene orf19.1604 encodes a zinc cluster transcription factor regulating filament development. Hyperactivation of orf19.1604, which we have named RHA1 for Regulator of Hyphal ...

Article GUID: 34849863

Author(s): Lismer A; Lambrot R; Lafleur C; Dumeaux V; Kimmins S;

In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally. We describe mouse model design to probe chromatin states in single mouse sperm and techniques to assess pre-implant ...

Article GUID: 34159325

Author(s): Costa ACBP; Omran RP; Law C; Dumeaux V; Whiteway M;

Candida albicans opaque cells release pheromones to stimulate cells of opposite mating type to activate their pheromone response pathway. Although this fungal pathogen shares orthologous proteins involved in the process with Saccharomyces cerevisiae, the pathway in each organism has unique characteristics. We have used GFP-tagged fusion proteins to invest ...

Article GUID: 33793759

Author(s): Lismer A, Dumeaux V, Lafleur C, Lambrot R, Brind'Amour J, Lorincz MC, Kimmins S

A father's lifestyle impacts offspring health; yet, the underlying molecular mechanisms remain elusive. We hypothesized that a diet that changes methyl donor availability will alter the sperm and embryo epigenomes to impact embryonic gene expression and development. Here, we demonstrate that a folate-deficient (FD) diet alters histone H3 lysine 4 trim ...

Article GUID: 33596408

Author(s): Lismer A; Siklenka K; Lafleur C; Dumeaux V; Kimmins S;

Advancing the molecular knowledge surrounding fertility and inheritance has become critical given the halving of sperm counts in the last 40 years, and the rise in complex disease which cannot be explained by genetics alone. The connection between both these trends may lie in alterations to the sperm epigenome and occur through environmental exposures. Ch ...

Article GUID: 33068438

Author(s): Tamming RJ, Dumeaux V, Jiang Y, Shafiq S, Langlois L, Ellegood J, Qiu LR, Lerch JP, Bérubé NG

Cell Rep. 2020 Jun 30;31(13):107838 Authors: Tamming RJ, Dumeaux V, Jiang Y, Shafiq S, Langlois L, Ellegood J, Qiu LR, Lerch JP, Bérubé NG

Article GUID: 32610139

Author(s): Jaju Bhattad G, Jeyarajah MJ, McGill MG, Dumeaux V, Okae H, Arima T, Lajoie P, Bérubé NG, Renaud SJ

Cell Death Dis. 2020 May 04;11(5):311 Authors: Jaju Bhattad G, Jeyarajah MJ, McGill MG, Dumeaux V, Okae H, Arima T, Lajoie P, Bérubé NG, Renaud SJ

Article GUID: 32366868

Author(s): Rakovitch E, Sutradhar R, Hallett M, Thompson AM, Gu S, Dumeaux V, Whelan TJ, Paszat L

Breast Cancer Res Treat. 2019 Jul 31;: Authors: Rakovitch E, Sutradhar R, Hallett M, Thompson AM, Gu S, Dumeaux V, Whelan TJ, Paszat L

Article GUID: 31368035