Author(s): Zhang C; Calderin JD; Hurst LR; Gokbayrak ZD; Hrabak MR; Balutowski A; Rivera-Kohr DA; Kazmirchuk TDD; Brett CL; Fratti RA;

Sphingolipids are essential in membrane trafficking and cellular homeostasis. Here, we show that sphingolipids containing very long-chain fatty acids (VLCFAs) promote homotypic vacuolar fusion in Saccharomyces cerevisiae. The elongase Elo3 adds the last two carbons to VLCFAs that are incorporated ...

Article GUID: 39307308

Author(s): Logan CJ; Staton CC; Oliver JT; Bouffard J; Kazmirchuk TDD; Magi M; Brett CL;

The budding yeast Saccharomyces cerevisiae is a proven model organism for elucidating conserved eukaryotic biology, but to date its extracellular vesicle (EV) biology is understudied. Here, we show yeast transmit information through the extracellular medium that increases survival when confronted with heat stress and demonstrate the EV-enriched samples me ...

Article GUID: 38711329

Author(s): Golden CK; Kazmirchuk TDD; McNally EK; El Eissawi M; Gokbayrak ZD; Richard JD; Brett CL;

Diverse physiology relies on receptor and transporter protein down-regulation and degradation mediated by ESCRTs. Loss-of-function mutations in human ESCRT genes linked to cancers and neurological disorders are thought to block this process. However, when homologous mutations are introduced into model organisms, cells thrive and degradation persists, sugg ...

Article GUID: 36215320

Author(s): Gokbayrak ZD; Patel D; Brett CL;

Vacuoles in plants and fungi play critical roles in cell metabolism and osmoregulation. To support these functions, vacuoles change their morphology, e.g. they fragment when these organisms are challenged with draught, high salinity or metabolic stress (e.g. acetate accumulation). In turn, morphology reflects an equilibrium between membrane fusion and fis ...

Article GUID: 35834522

Author(s): Karim MA, Samyn DR, Mattie S, Brett CL

Traffic. 2018 02;19(2):138-149 Authors: Karim MA, Samyn DR, Mattie S, Brett CL

Article GUID: 29135058

Author(s): Karim MA, Brett CL

Mol Biol Cell. 2018 02 01;29(3):317-325 Authors: Karim MA, Brett CL

Article GUID: 29212874

Author(s): Karim MA, McNally EK, Samyn DR, Mattie S, Brett CL

Dev Cell. 2018 10 08;47(1):80-97.e6 Authors: Karim MA, McNally EK, Samyn DR, Mattie S, Brett CL

Article GUID: 30269949

Author(s): Karim MA, Samyn DR, Brett CL

Methods Mol Biol. 2019;1860:289-301 Authors: Karim MA, Samyn DR, Brett CL

Article GUID: 30317513

Author(s): Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL

Methods Mol Biol. 2019;1860:361-377 Authors: Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL

Article GUID: 30317518

Author(s): McNally EK, Brett CL

Nat Commun. 2018 12 18;9(1):5358 Authors: McNally EK, Brett CL

Article GUID: 30560896