Author(s): Sultana S; Abdullah M; Li J; Hochstrasser M; Kachroo AH;

Yeast and humans share thousands of genes despite a billion years of evolutionary divergence. While many human genes can functionally replace their yeast counterparts, nearly half of the tested shared genes cannot. For example, most yeast proteasome subunits are "humanizable", except subunits comprising the ß-ring core, including ß2c (HsPSMB7, a constitut ...

Article GUID: 37364278

Author(s): Abdullah M; Greco BM; Laurent JM; Garge RK; Boutz DR; Vandeloo M; Marcotte EM; Kachroo AH;

A major challenge to rationally building multi-gene processes in yeast arises due to the combinatorics of combining all of the individual edits into the same strain. Here, we present a precise and multi-site genome editing approach that combines all edits without selection markers using CRISPR-Ca ...

Article GUID: 37323580

Author(s): Kachroo AH; Vandeloo M; Greco BM; Abdullah M;

For decades, budding yeast, a single-cellular eukaryote, has provided remarkable insights into human biology. Yeast and humans share several thousand genes despite morphological and cellular differences and over a billion years of separate evolution. These genes encode critical cellular processes, the failure of which in humans results in disease. Althoug ...

Article GUID: 35661208