1 Department of Pharmacology and Physiology, Université de Montréal, Montréal, QC, Canada. Electronic address: vincent.herve@umontreal.ca.
2 Department of Pharmacology and Physiology, Université de Montréal, Montréal, QC, Canada.
3 Department of Electrical and Computer Engineering, Concordia University, Montréal, QC, Canada.
4 Department of Pharmacology and Physiology, Université de Montréal, Montréal, QC, Canada. Electronic address: jonathan.brouillette@umontreal.ca.

In Alzheimer's disease, there is an imbalance in neurotransmitter release and altered neuronal activation. Here, we present a protocol approach to analyze neuronal activity by combining local field potential (LFP) recording with microdialysis within the same animal. We describe steps for measuring glutamate and GABA levels following hippocampal amyloid-beta oligomer (Aßo) injections in rats. We then detail procedures for assembling the electrode and cannula, surgical implantation and simultaneous in vivo LFP recording, interstitial fluid collection, and Aßo injections.