1 School of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany.2 Unit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.3 Applied Analytical Chemistry, University of Duisburg-Essen, Essen, Germany.4 Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.5 Neonatal Research Group, Health Research Institute La Fe (IIS La Fe), Valencia, Spain.6 Department of Chemistry and Biochemistry, Concordia University, Montreal, Canada.7 School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.8 Department of Analytical Chemistry, University of Valencia, Burjassot, Spain.9 Mass Spectrometry Core Facility, Department of Chemistry, Columbia University, New York, NY, USA.10 School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.11 De

Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.