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Effects of chronodisruption and alcohol consumption on gene expression in reward-related brain areas in female rats

Author(s): Meyer C; Schoettner K; Amir S;

Circadian dysfunction caused by exposure to aberrant light-dark conditions is associated with abnormal alcohol consumption in humans and animal models. Changes in drinking behavior have been linked to alterations in clock gene expression in reward-related brain areas, which could be attributed to either the effect of chronodisruption or alcohol. To date, ...

Article GUID: 39624490


Transcriptomics identify the triggering of citrate export as the key event caused by manganese deficiency in Aspergillus niger

Author(s): Fekete E; Bíró V; Márton A; Bakondi-Kovács I; Sándor E; Kovács B; Geoffrion N; Tsang A; Kubicek CP; Karaffa L;

For over a century, the filamentous Ascomycete fungus Aspergillus niger has played a pivotal role in the industrial production of citric acid. A critical fermentation parameter that sustains high-yield citric acid accumulation is the suboptimal concentration of manganese(II) ions in the culture b ...

Article GUID: 39377610


CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersonii

Author(s): Singh V; Raheja Y; Basotra N; Sharma G; Tsang A; Chadha BS;

Background: The filamentous fungus Rasamsonia emersonii has immense potential to produce biorefinery relevant thermostable cellulase and hemicellulase enzymes using lignocellulosic biomass. Previously in our lab, a hyper-cellulase producing strain of R. emersonii was developed through classical breeding and system biology approaches. ACE1, a pivotal trans ...

Article GUID: 37658430


Characterization of the heterotrimeric G protein gene families in Triticum aestivum and related species

Author(s): Gawande ND; Hamiditabar Z; Brunetti SC; Gulick PJ;

This study characterizes the heterotrimeric G protein gene families in Triticum aestivum, their tissue-specific expression patterns during development and in response to biotic and abiotic stress conditions. There are three Ga genes, three Gß and 12 G? genes, totaling 18 genes encoding heterotrimeric G proteins in the hexaploid wheat genome. Each haploid ...

Article GUID: 35463045


ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance

Author(s): Lismer A; Lambrot R; Lafleur C; Dumeaux V; Kimmins S;

In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally. We describe mouse model design to probe chromatin states in single mouse sperm and techniques to assess pre-implant ...

Article GUID: 34159325


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