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Heterologous Expression of Thermostable Endoglucanases from Rasamsonia emersonii: A Paradigm Shift in Biomass Hydrolysis

Author(s): Raheja Y; Singh V; Gaur VK; Tsang A; Chadha BS;

In this study, two thermostable endoglucanases (Rem_GH5EG and Rem_GH7EG) from Rasamsonia emersonii were heterologously expressed in Pichia pastoris and characterized to evaluate their potential for industrial biomass saccharification. Rem_GH5EG demonstrated markedly superior catalytic efficiency toward barley ß-glucan (kcat/Km = 6.3 × 10-3/mg mL/min), whi ...

Article GUID: 40418313


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