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Comparative genomic analysis of thermophilic fungi reveals convergent evolutionary adaptations and gene losses

Author(s): Steindorff AS; Aguilar-Pontes MV; Robinson AJ; Andreopoulos B; LaButti K; Kuo A; Mondo S; Riley R; Otillar R; Haridas S; Lipzen A; Grimwood ...

Thermophily is a trait scattered across the fungal tree of life, with its highest prevalence within three fungal families (Chaetomiaceae, Thermoascaceae, and Trichocomaceae), as well as some member ...

Article GUID: 39266695


Developing endophytic Penicillium oxalicum as a source of lignocellulolytic enzymes for enhanced hydrolysis of biorefinery relevant pretreated rice straw

Author(s): Sharma G; Kaur B; Raheja Y; Kaur A; Singh V; Basotra N; Di Falco M; Tsang A; Chadha BS;

Endophytic fungi, as plant symbionts, produce an elaborate array of enzymes for efficient disintegration of lignocellulosic biomass into constituent monomeric sugars, making them novel source of lignocellulolytic CAZymes with immense potential in future bio ...

Article GUID: 39249151


Transcriptional and secretome analysis of Rasamsonia emersonii lytic polysaccharide mono-oxygenases

Author(s): Raheja Y; Singh V; Kumar N; Agrawal D; Sharma G; Di Falco M; Tsang A; Chadha BS;

The current study is the first to describe the temporal and differential transcriptional expression of two lytic polysaccharide monooxygenase (LPMO) genes of Rasamsonia emersonii in response to various carbon sources. The mass spectrometry based secretome a ...

Article GUID: 39167166


Expansion of Auxiliary Activity Family 5 sequence space via biochemical characterization of six new copper radical oxidases

Author(s): Fong JK; Mathieu Y; Vo MT; Bellemare A; Tsang A; Brumer H;

Bacterial and fungal copper radical oxidases (CROs) from Auxiliary Activity Family 5 (AA5) are implicated in morphogenesis and pathogenesis. The unique catalytic properties of CROs also make these enzymes attractive biocatalysts for the transformation of sm ...

Article GUID: 38953370


Genome and secretome insights: unravelling the lignocellulolytic potential of Myceliophthora verrucosa for enhanced hydrolysis of lignocellulosic biomass

Author(s): Sharma G; Kaur B; Singh V; Raheja Y; Falco MD; Tsang A; Chadha BS;

Lignocellulolytic enzymes from a novel Myceliophthora verrucosa (5DR) strain was found to potentiate the efficacy of benchmark cellulase during saccharification of acid/alkali treated bagasse by ~ 2.24 fold, indicating it to be an important source of auxili ...

Article GUID: 38676717


Substrate specificity mapping of fungal CAZy AA3_2 oxidoreductases

Author(s): Zhao H; Karppi J; Mototsune O; Poshina D; Svartström J; Nguyen TTM; Vo TM; Tsang A; Master E; Tenkanen M; ...

Background: Oxidative enzymes targeting lignocellulosic substrates are presently classified into various auxiliary activity (AA) families within the carbohydrate-active enzyme (CAZy) database. Amon ...

Article GUID: 38539167


A thermostable and inhibitor resistant β-glucosidase from Rasamsonia emersonii for efficient hydrolysis of lignocellulosics biomass

Author(s): Raheja Y; Singh V; Sharma G; Tsang A; Chadha BS;

The present study reports a highly thermostable ß-glucosidase (GH3) from Rasamsonia emersonii that was heterologously expressed in Pichia pastoris. Extracellular ß-glucosidase was purified to homogeneity using single step affinity chromatography with molecu ...

Article GUID: 38470501


Comparative analysis of functional diversity of rumen microbiome in bison and beef heifers

Author(s): Nguyen TTM; Badhan AK; Reid ID; Ribeiro G; Gruninger R; Tsang A; Guan LL; McAllister T;

Ruminants play a key role in the conversion of cellulolytic plant material into high-quality meat and milk protein for humans. The rumen microbiome is the driver of this conversion, yet there is little information on how gene expression within the microbiom ...

Article GUID: 38054735


Functional screening pipeline to uncover laccase-like multicopper oxidase enzymes that transform industrial lignins

Author(s): Sharan AA; Bellemare A; DiFalco M; Tsang A; Vuong TV; Edwards EA; Master ER;

Laccase-like multicopper oxidases are recognized for their potential to alter the reactivity of lignins for application in value-added products. Typically, model compounds are employed to discover such enzymes; however, they do not represent the complexity ...

Article GUID: 38000639


Identification of a Conserved Transcriptional Activator-Repressor Module Controlling the Expression of Genes Involved in Tannic Acid Degradation and Gallic Acid Utilization in Aspergillus niger

Author(s): Arentshorst M; Falco MD; Moisan MC; Reid ID; Spaapen TOM; van Dam J; Demirci E; Powlowski J; Punt PJ; Tsang A; Ram AFJ; ...

Tannic acid, a hydrolysable gallotannin present in plant tissues, consists of a central glucose molecule esterified with gallic acid molecules. Some microorganisms, including several Aspergillus sp ...

Article GUID: 37744122


Utilization of ferulic acid in Aspergillus niger requires the transcription factor FarA and a newly identified Far-like protein (FarD) that lacks the canonical Zn(II)2Cys6 domain

Author(s): Arentshorst M; Reijngoud J; van Tol DJC; Reid ID; Arendsen Y; Pel HJ; van Peij NNME; Visser J; Punt PJ; Tsang A; Ram AFJ; ...

The feruloyl esterase B gene (faeB) is specifically induced by hydroxycinnamic acids (e.g. ferulic acid, caffeic acid and coumaric acid) but the transcriptional regulation network involved in faeB ...

Article GUID: 37746181


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