Author(s): Sgro M; Reid ID; Arentshorst M; Ram AFJ; Tsang A;

Many microorganisms are able to use plant-derived aromatic and cyclic compounds like the common plant secondary metabolite quinic acid as carbon and energy sources. In fungi, three enzymatic steps convert quinic acid into the common intermediate protocatechuic acid, which is then further converted into TCA cycle intermediates. The genes encoding these thr ...

Article GUID: 40853219

Author(s): Semper C; Pham TTM; Ram S; Palys S; Evdokias G; Ouedraogo JP; Moisan MC; Geoffrion N; Reid I; Di Falco M; Bailey Z; Tsang A; Benoit-Gelber I; Savchenko A;

Genomics analysis confirmed the status of filamentous fungi as a rich source of novel secondary metabolites; however, the discovery of these compounds is hampered by the cryptic nature of their biosynthetic pathways under laboratory conditions. Consequently, despite substantial research effort ov ...

Article GUID: 40852424

Author(s): Raheja Y; Singh V; Gaur VK; Sharma G; Tsang A; Chadha BS;

GH7 cellobiohydrolases (CBH1s) are essential for depolymerizing crystalline cellulose, yet the hypercellulolytic thermophile Rasamsonia emersonii secretes them only in low amounts, leaving a gap in its native enzyme cocktail. To see whether a cognate CBH1 could fill this gap and how it stacks up against the industrial workhorse strain Trichoderma reesei C ...

Article GUID: 40622460

Author(s): Raheja Y; Singh V; Gaur VK; Tsang A; Chadha BS;

In this study, two thermostable endoglucanases (Rem_GH5EG and Rem_GH7EG) from Rasamsonia emersonii were heterologously expressed in Pichia pastoris and characterized to evaluate their potential for industrial biomass saccharification. Rem_GH5EG demonstrated markedly superior catalytic efficiency toward barley ß-glucan (kcat/Km = 6.3 × 10-3/mg mL/min), whi ...

Article GUID: 40418313

Author(s): Chadha BS; Kaur B; Basotra N; Tsang A; Pandey A;

No abstract available

Article GUID: 39447502

Author(s): Fekete E; Bíró V; Márton A; Bakondi-Kovács I; Sándor E; Kovács B; Geoffrion N; Tsang A; Kubicek CP; Karaffa L;

For over a century, the filamentous Ascomycete fungus Aspergillus niger has played a pivotal role in the industrial production of citric acid. A critical fermentation parameter that sustains high-yield citric acid accumulation is the suboptimal concentration of manganese(II) ions in the culture b ...

Article GUID: 39377610

Author(s): Steindorff AS; Aguilar-Pontes MV; Robinson AJ; Andreopoulos B; LaButti K; Kuo A; Mondo S; Riley R; Otillar R; Haridas S; Lipzen A; Grimwood J; Schmutz J; Clum A; Reid ID; Moisan MC; Butler G; Nguyen TTM; Dewar K; Conant G; Drula E; Henri ...

Thermophily is a trait scattered across the fungal tree of life, with its highest prevalence within three fungal families (Chaetomiaceae, Thermoascaceae, and Trichocomaceae), as well as some members of the phylum Mucoromycota. We examined 37 thermophilic and thermotolerant species and 42 mesophil ...

Article GUID: 39266695

Author(s): Sharma G; Kaur B; Raheja Y; Kaur A; Singh V; Basotra N; Di Falco M; Tsang A; Chadha BS;

Endophytic fungi, as plant symbionts, produce an elaborate array of enzymes for efficient disintegration of lignocellulosic biomass into constituent monomeric sugars, making them novel source of lignocellulolytic CAZymes with immense potential in future biorefineries. The present study reports lignocellulolytic enzymes production potential of an endophyti ...

Article GUID: 39249151

Author(s): Raheja Y; Singh V; Kumar N; Agrawal D; Sharma G; Di Falco M; Tsang A; Chadha BS;

The current study is the first to describe the temporal and differential transcriptional expression of two lytic polysaccharide monooxygenase (LPMO) genes of Rasamsonia emersonii in response to various carbon sources. The mass spectrometry based secretome analysis of carbohydrate active enzymes (CAZymes) expression in response to different carbon sources ...

Article GUID: 39167166

Author(s): Fong JK; Mathieu Y; Vo MT; Bellemare A; Tsang A; Brumer H;

Bacterial and fungal copper radical oxidases (CROs) from Auxiliary Activity Family 5 (AA5) are implicated in morphogenesis and pathogenesis. The unique catalytic properties of CROs also make these enzymes attractive biocatalysts for the transformation of small molecules and biopolymers. Despite a recent increase in the number of characterized AA5 members, ...

Article GUID: 38953370

Author(s): Sharma G; Kaur B; Singh V; Raheja Y; Falco MD; Tsang A; Chadha BS;

Lignocellulolytic enzymes from a novel Myceliophthora verrucosa (5DR) strain was found to potentiate the efficacy of benchmark cellulase during saccharification of acid/alkali treated bagasse by ~ 2.24 fold, indicating it to be an important source of auxiliary enzymes. The De-novo sequencing and analysis of M. verrucosa genome (31.7 Mb) revealed to encode ...

Article GUID: 38676717