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Miniaturized scalable arrayed CRISPR screening in primary cells enables discovery at the single donor resolution

Author(s): Patel MA; Boribong BP; Sinha H; Xiao B; Xie K; Vo PQN; Chin AB; Ellouzi A; Little SR; Shih S; Wu H; Muller WJ; Hirukawa A;

High-efficiency gene editing in primary human cells is critical for advancing therapeutic development and functional genomics, yet conventional electroporation platforms often require high cell input and are poorly suited to parallelized experiments. Here we introduce a next-generation digital mi ...

Article GUID: 40790054


A Digital Microfluidic Platform for the Microscale Production of Functional Immune Cell Therapies

Author(s): Little SR; Rahbari N; Hajiaghayi M; Gholizadeh F; Cloarec-Ung FM; Phillips J; Sinha H; Hirukawa A; Knapp DJHF; Darlington PJ; Shih SCC;

Genetically engineering human immune cells has been shown to be an effective approach for developing novel cellular therapies to treat a wide range of diseases. To expand the scope of these cellular therapies while solving persistent challenges, extensive research and development is still require ...

Article GUID: 40390294


An Automated Induction Microfluidics System for Synthetic Biology.

Author(s): Husser MC, Vo PQN, Sinha H, Ahmadi F, Shih SCC

ACS Synth Biol. 2018 03 16;7(3):933-944 Authors: Husser MC, Vo PQN, Sinha H, Ahmadi F, Shih SCC

Article GUID: 29516725


An automated microfluidic gene-editing platform for deciphering cancer genes.

Author(s): Sinha H, Quach ABV, Vo PQN, Shih SCC

Lab Chip. 2018 07 24;18(15):2300-2312 Authors: Sinha H, Quach ABV, Vo PQN, Shih SCC

Article GUID: 29989627


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