Author(s): Raheja Y; Singh V; Gaur VK; Sharma G; Tsang A; Chadha BS;

GH7 cellobiohydrolases (CBH1s) are essential for depolymerizing crystalline cellulose, yet the hypercellulolytic thermophile Rasamsonia emersonii secretes them only in low amounts, leaving a gap in its native enzyme cocktail. To see whether a cognate CBH1 could fill this gap and how it stacks up against the industrial workhorse strain Trichoderma reesei C ...

Article GUID: 40622460

Author(s): Raheja Y; Singh V; Gaur VK; Tsang A; Chadha BS;

In this study, two thermostable endoglucanases (Rem_GH5EG and Rem_GH7EG) from Rasamsonia emersonii were heterologously expressed in Pichia pastoris and characterized to evaluate their potential for industrial biomass saccharification. Rem_GH5EG demonstrated markedly superior catalytic efficiency toward barley ß-glucan (kcat/Km = 6.3 × 10-3/mg mL/min), whi ...

Article GUID: 40418313