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Transcriptional and secretome analysis of Rasamsonia emersonii lytic polysaccharide mono-oxygenases

Author(s): Raheja Y; Singh V; Kumar N; Agrawal D; Sharma G; Di Falco M; Tsang A; Chadha BS;

The current study is the first to describe the temporal and differential transcriptional expression of two lytic polysaccharide monooxygenase (LPMO) genes of Rasamsonia emersonii in response to various carbon sources. The mass spectrometry based secretome a ...

Article GUID: 39167166


Lignocellulolytic enzymes from Aspergillus allahabadii for efficient bioconversion of rice straw into fermentable sugars and biogas

Author(s): Sharma G; Kaur B; Raheja Y; Agrawal D; Basotra N; Di Falco M; Tsang A; Singh Chadha B;

The study was aimed at developing lignocellulolytic strain capable of efficient hydrolysis of mild alkali deacetylated (MAD) rice straw. The valorisation of lignin rich black liquor obtained during pre-treatment of rice straw into biogas was also evaluated. ...

Article GUID: 35753566


Economizing the lignocellulosic hydrolysis process using heterologously expressed auxiliary enzymes feruloyl esterase D (CE1) and β-xylosidase (GH43) derived from thermophilic fungi Scytalidium thermophilum

Author(s): Agrawal D; Tsang A; Chadha BS;

Two lignocellulolytic accessory enzymes, feruloyl esterase D (FAED_SCYTH) and ß-xylosidase (XYL43B_SCYTH) were cloned and produced in the Pichia pastoris X33 as host. The molecular weight of recombinant enzymes FAED_SCYTH and XYL43B_SCYTH were ~ 31 and 40 k ...

Article GUID: 34293687


Discovery and Expression of Thermostable LPMOs from Thermophilic Fungi for Producing Efficient Lignocellulolytic Enzyme Cocktails.

Author(s): Agrawal D, Basotra N, Balan V, Tsang A, Chadha BS

Appl Biochem Biotechnol. 2019 Dec 02;: Authors: Agrawal D, Basotra N, Balan V, Tsang A, Chadha BS

Article GUID: 31792786


Characterization of a novel Lytic Polysaccharide Monooxygenase from Malbranchea cinnamomea exhibiting dual catalytic behavior

Author(s): Basotra N; Dhiman SS; Agrawal D; Sani RK; Tsang A; Chadha BS;

A novel Lytic Polysaccharide Monooxygenase (LPMO) family AA9 (PMO9A_MALCI) protein from thermophilic fungus Malbranchea cinnamomea was cloned and expressed in Pichia pastoris. The expressed protein was purified to homogeneity using ion exchange and hydropho ...

Article GUID: 31054382


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